제품 수집
제품 세부정보
Synonym
PVR, FLJ25946, PVS, CD155, TAGE4, HVED, NECL5
Source
Human CD155, Fc Tag (CD5-H5251) is expressed from human 293 cells (HEK293). It contains AA Trp 21 - Asn 343 (Accession # NP_006496.2).
Predicted N-terminus: Trp 21
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a human IgG1 Fc tag at the C-terminus.
The protein has a calculated MW of 61.7 kDa. The protein migrates as 70-90 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin
Less than 0.01 EU per μg by the LAL method / rFC method.
Purity
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
ACRO Quality Management System
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데이터 표시
SDS-PAGE
Human CD155, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Human CD155, Fc Tag (Cat. No. CD5-H5251) is more than 90% and the molecular weight of this protein is around 135-165 kDa verified by SEC-MALS.
Report
Bioactivity-ELISA
Immobilized Human TIGIT, His Tag at 2 μg/mL (100 μL/well) can bind Human CD155, Fc Tag (Cat. No. CD5-H5251) with a linear range of 8-320 ng/mL (QC tested).
Protocol
Serial dilutions of Human TIGIT Neutralizing antibody were added into Human CD155, Fc Tag (Cat. No. CD5-H5251): Biotinylated Human TIGIT, Fc,Avitag (Cat. No. TIT-H82F1) binding reactions. The half maximal inhibitory concentration (IC50) is 0.06065 μg/mL (Routinely tested).
Protocol
Bioactivity-SPR
Human CD155, Fc Tag (Cat. No. CD5-H5251) captured on CM5 chip via Anti-human IgG Fc antibodies surface can bind Canine DNAM-1, His Tag with an affinity constant of 31.9 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Protocol
Human CD155, Fc Tag (Cat. No. CD5-H5251) captured on CM5 chip via Anti-human IgG Fc antibodies surface can bind Mouse DNAM-1, His Tag (Cat. No. DN1-M52H5) with an affinity constant of 0.816 μM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Protocol
Bioactivity-BLI
Loaded Human CD155, Fc Tag (Cat. No. CD5-H5251) on Protein A Biosensor, can bind Human TIGIT, His Tag with an affinity constant of 0.23 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
Loaded Human CD155, Fc Tag (Cat. No. CD5-H5251) on Protein A Biosensor, can bind Cynomolgus DNAM-1, His Tag (Cat. No. DN1-C52H9) with an affinity constant of 0.88 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
Bioactivity-FACS
FACS assay shows that Human CD155, Fc Tag (Cat. No. CD5-H5251) can bind to 293T cell overexpressing human TIGIT. The concentration of CD155 is 1 μg/mL (Routinely tested).
Protocol
FACS analysis shows that the binding of Human CD155, Fc Tag (Cat. No. CD5-H5251) to 293T overexpressing TIGIT was inhibited by increasing concentration of neutralizing Anti-Human TIGIT MAb. The concentration of CD155 used is 1 μg/mL. The IC50 is 0.09422 μg/mL (Routinely tested).
Protocol
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배경 소개
CD155/PVR was originally isolated based on its ability to mediate polio virus attachment to host cells. The fulllength (or CD155 alpha isoform) is synthesized as a 417 amino acid (aa) precursor that contains a 20 aa signal sequence, a 323 aa extracellular region, a 24 aa TM segment and a 50 aa cytoplasmic tail. The extracellular region contains one N terminal V type and two C2 type Ig like domains.
CD155 is a transmembrane protein with 3 extracellular immunoglobulin-like domains, D1-D3, where D1 is recognized by the virus. Low resolution structures of CD155 complexed with poliovirus have been obtained using electron microscopy while a high resolution structures of theectodomain D1 and D2 of CD155 were solved by x-ray crystallography.
프론티어 진행
의약품 개발 진행- 영어 이름：
Poliovirus receptor
- 카테고리：
- 상장 의약품 수：
0 상세
- 임상 의약품 수：
2 상세
- 최고 개발 단계：
Phase 2 Clinical
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