Product Details
Synonym
CCL2, C-C motif chemokine 2, MCP-1, MCAF
Source
Human CCL2, Fc Tag (CC2-H5255) is expressed from human 293 cells (HEK293). It contains AA Gln 24 - Thr 99 (Accession # P13500-1).
Predicted N-terminus: Gln 24
Request for sequenceMolecular Characterization

Other Tags and Version Biotin & Other Labeled Version
This protein carries a human IgG1 Fc tag at the C-terminus.
The protein has a calculated MW of 35.1 kDa. The protein migrates as 40-50 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin
Less than 1.0 EU per μg by the LAL method / rFC method.
Purity
>90% as determined by SDS-PAGE.
>95% as determined by SEC-MALS.
Formulation
Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, 25 mM Arginine, 150 mM NaCl, pH7.5 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Performance Data
SDS-PAGE

Human CCL2, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.
SEC-MALS

The purity of Human CCL2, Fc Tag (Cat. No. CC2-H5255) is more than 95% and the molecular weight of this protein is around 68-88 kDa verified by SEC-MALS.
Report
Bioactivity-ELISA

Immobilized Human CCL2, Fc Tag (Cat. No. CC2-H5255) at 5 μg/mL (100 μL/well) can bind Human CCR2 Full Length Protein, Flag,His Tag (Cat. No. CC2-H52D4) with a linear range of 0.005-0.313 μg/mL (QC tested).
Protocol
Immobilized Human CCL2, Fc Tag (Cat. No. CC2-H5255) at 5 μg/mL (100 μL/well) can bind Anti-MCP-1 / CCL2 Antibody, Mouse Fc (1B7) with a linear range of 0.2-5 ng/mL (Routinely tested).
Protocol
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Background
Recent Advances
- English Name:
C-C motif chemokine 2
- Category:
- Approved Drugs:
1 Details
- Drugs in Clinical Trials:
3 Details
- Highest Development Stage:
Approved
전화
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