Product Details
Synonym
APRIL, TNFSF13, TALL-2, TRDL-1, CD256, TALL2, ZTNF2
Source
Cynomolgus APRIL, His,Flag Tag (APL-C52D2) is expressed from human 293 cells (HEK293). It contains AA Gln 111 - Leu 250 (Accession # XP_005582818.1) trimer Design.
Predicted N-terminus: His
Molecular Characterization
This protein carries a polyhistidine tag at the N-terminus, followed by a flag tag.
The protein has a calculated MW of 50.4 kDa. The protein migrates as 55-65 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin
Less than 0.01 EU per μg by the LAL method / rFC method.
Purity
>95% as determined by SDS-PAGE.
>95% as determined by SEC-MALS.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Performance Data
SDS-PAGE

Cynomolgus APRIL, His,Flag Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS

The purity of Cynomolgus APRIL, His,Flag Tag (Cat. No. APL-C52D2) is more than 95% and the molecular weight of this protein is around 57-69 kDa verified by SEC-MALS.
Report
Bioactivity-ELISA

Immobilized Cynomolgus APRIL, His,Flag Tag (Cat. No. APL-C52D2) at 1 μg/mL (100 μL/well) can bind Human TACI (R119A, R122A), Fc Tag (Cat. No. TAI-H5253) with a linear range of 2-13 ng/mL (QC tested).
Protocol
Immobilized Cynomolgus APRIL, His,Flag Tag (Cat. No. APL-C52D2) at 1 μg/mL (100 μL/well) can bind Human BCMA, Fc Tag (HPLC verified) (Cat. No. BC7-H5254) with a linear range of 0.1-1 ng/mL (Routinely tested).
Protocol
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Background
Recent Advances
- English Name:
Tumor necrosis factor ligand superfamily member 13
- Category:
- Approved Drugs:
2 Details
- Drugs in Clinical Trials:
30 Details
- Highest Development Stage:
Approved
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