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Product Details
Synonym
Apolipoprotein E, APOE, Apo-E
Source
Human APOE3 Protein, Tag Free (APE-H5216) is expressed from human 293 cells (HEK293). It contains AA Lys 19 - His 317 (Accession # P02649-1 ).
Predicted N-terminus: Lys 19
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries no "tag".
The protein has a calculated MW of 35.0 kDa. The protein migrates as 37-40 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin
Less than 0.01 EU per μg by the LAL method / rFC method.
Purity
>90% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Performance Data
SDS-PAGE
Human APOE3 Protein, Tag Free on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Human APOE3 Protein, Tag Free (Cat. No. APE-H5216) is more than 90% and the molecular weight of this protein is around 142-168 kDa verified by SEC-MALS.
Report
Bioactivity-FACS
3e5 of HeLa cells were co-incubated with 50 nM Cy3-labeled LNPs-siRNA in the presence or absence of 3 μg/mL Human Apolipoprotein E / APOE3 Protein, Tag Free (MALS verified) (ACRO Biosystems, Cat. No. APE-H5216), washed and analyzed with FACS. PE signal was used to evaluate the expression of Cy3+ HeLa cells (Routinely tested).
Protocol
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Background
Recent Advances
Drug Development Progress- English Name：
Apolipoprotein E
- Category：
- Approved Drugs：
0 Details
- Drugs in Clinical Trials：
3 Details
- Highest Development Stage：
Phase 2 Clinical
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