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Product Details
Synonym
TNFRSF9, 4-1BB, CD137, CDw137, ILA
Source
Human 4-1BB, Fc Tag (41B-H5258) is expressed from human 293 cells (HEK293). It contains AA Leu 24 - Gln 186 (Accession # NP_001552.2).
Predicted N-terminus: Leu 24
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a human IgG1 Fc tag at the C-terminus.
The protein has a calculated MW of 43.4 kDa. The protein migrates as 50-60 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin
Less than 0.01 EU per μg by the LAL method / rFC method.
Purity
>90% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Formulation
Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Performance Data
SDS-PAGE
Human 4-1BB, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Human 4-1BB, Fc Tag (Cat. No. 41B-H5258) is more than 90% and the molecular weight of this protein is around 90-110 kDa verified by SEC-MALS.
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Bioactivity-ELISA
Immobilized Human 4-1BB, Fc Tag (Cat. No. 41B-H5258) at 0.1 μg/mL (100 μL/well) can bind Biotinylated Human 4-1BB Ligand, Fc,Avitag (Cat. No. 41L-H82F9) with a linear range of 0.4-6 ng/mL (QC tested).
Protocol
Immobilized Human 4-1BB, Fc Tag (Cat. No. 41B-H5258) at 1 μg/mL (100 μL/well) can bind Anti-Human 4-1BB MAb, Human IgG4 with a linear range of 0.2-3 ng/mL (Routinely tested).
Protocol
Immobilized Human 4-1BB, Fc Tag (Cat. No. 41B-H5258) at 1 μg/mL (100 μL/well) can bind Utomilumab with a linear range of 2-31 ng/mL (Routinely tested).
Protocol
Bioactivity-BLI
Loaded Human 4-1BB, Fc Tag (Cat. No. 41B-H5258) on Protein A Biosensor, can bind Human 4-1BB Ligand, His Tag with an affinity constant of 1.3 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
Bioactivity-FACS
Flow Cytometry assay shows that Human 4-1BB, Fc Tag (Cat. No. 41B-H5258) can bind to 293T cells overexpressing Human 4-1BBL. The concentration of 4-1BB used is 0.1 μg/mL (Routinely tested).
Protocol
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Background
Recent Advances
Drug Development Progress- English Name：
Tumor necrosis factor receptor superfamily member 9
- Category：
- Approved Drugs：
0 Details
- Drugs in Clinical Trials：
77 Details
- Highest Development Stage：
Phase 3 Clinical
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