제품 수집
제품 세부정보
Synonym
TNFRSF9, 4-1BB, CD137, CDw137, ILA
Source
Cynomolgus / Rhesus macaque 4-1BB, His Tag (41B-C52H4) is expressed from human 293 cells (HEK293). It contains AA Leu 24 - Gln 186 (Accession # XP_005544945.1). In the region Leu 24 - Gln 186, the AA sequence of Cynomolgus and Rhesus macaque 4-1BB are homologus.
Predicted N-terminus: Leu 24
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 19.2 kDa. The protein migrates as 30-40 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin
Less than 0.01 EU per μg by the LAL method / rFC method.
Purity
>90% as determined by SDS-PAGE.
>95% as determined by SEC-MALS.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
ACRO Quality Management System
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데이터 표시
SDS-PAGE
Cynomolgus / Rhesus macaque 4-1BB, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Cynomolgus / Rhesus macaque 4-1BB, His Tag (Cat. No. 41B-C52H4) is more than 95% and the molecular weight of this protein is around 25-35 kDa verified by SEC-MALS.
Report
Bioactivity-ELISA
Immobilized Cynomolgus / Rhesus macaque 4-1BB, His Tag (Cat. No. 41B-C52H4) at 1 μg/mL (100 μL/well) can bind Human 4‑1BB Ligand, Fc Tag with a linear range of 0.05-0.5 ng/mL (QC tested).
Protocol
Bridging ELISA
Validated with Bispecific Antibody.
Immobilized Cynomolgus / Rhesus macaque 4-1BB, His Tag (Cat. No. 41B-C52H4) at 1 μg/mL, add increasing concentrations of Acasunlimab and then add Biotinylated Cynomolgus / Rhesus macaque PD-L1, His, Avitag (Cat. No. PDL-C82E8) at 0.2 μg/mL. Detection was performed using HRP-conjugated streptavidin with sensitivity of 0.15 ng/mL (Routinely tested).
Bioactivity-BLI
Loaded Human 4-1BB Ligand (71-254), Fc Tag (active trimer) (MALS verified) on Protein A Biosensor, can bind Cynomolgus / Rhesus macaque 4-1BB, His Tag (Cat. No. 41B-C52H4) with an affinity constant of 69.8 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
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배경 소개
프론티어 진행
의약품 개발 진행- 영어 이름：
Tumor necrosis factor receptor superfamily member 9
- 카테고리：
- 상장 의약품 수：
0 상세
- 임상 의약품 수：
77 상세
- 최고 개발 단계：
Phase 3 Clinical
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전화
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