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Product Specifications
Assay TypeFACSAnalyteTNF-αFormat100 testsReactivityHumanRegulatory StatusRUOSensitivity0.78 pg/mLStandard Curve Range9.8-10000 pg/mLAssay Time3 hrSuitable Sample TypeHuman serum, Human plasma, Cell culture supernatantsSample volume30 μLProduct Overview
Multiplex Bead Assay platform allows flow cytometry based on beads with different size and color to quantity multiple soluble cell release factor from a single sample. The performance of this platform has been optimized for specific analysis in cell culture supernatants, plasma and serum samples. Compared to traditional single-analyte methods, it offers significant advantages, including lower sample volume requirements, simpler operation, more broad dynamic range and so on.
We offer a range of individual reagents for each factor, giving you the flexibility to select and combine them according to your specific research requirements.
Application
This Panel (Flow Cytometry Bead Assay) gives quantitative results of Tumor necrosis factor alpha (TNF-α) with a sample testing. The performance of this panel has been optimized for specific analysis of cytokines.
It is for research use only.
Storage
1. Unopened buffer should be stored at 2℃-8℃ upon receiving.
2. Find the expiration date on the outside packaging and do not use reagents past their expiration date.
3. The shelf life is 30 days from the date of opening.
Materials Provided
IDComponentsSizeFMBc010-02Anti-human TNF-α Antibody-coupled APC-beads-D1350 tests×2TNA-PFM494PE-Labeled Monoclonal Anti-TNF-alpha Antibody, human IgG1 (16H5)50 tests×2IL2-H51P3Calibrator(lL-2,IL-4,IL-6,IL-10,TNF-α,IFN-γ)5 μg (for each)×2FMBf001-02APC Positive Control-010.5 mLFMBf001-01PE Positive Control-010.5 mLFCMP-0296-well Sealing film2 piecesFCMP-0196-Well V-Bottom Assay Plate1 plateFCMB-03Assay Buffer-0150 mL×2FCMB-04Wash Buffer-0150 mL×2ACRO Quality Management System
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Performance Data
Typical Data
Please refer to DS document for the assay protocol.
A double logarithmic fitting curve was plotted for TNF-α. The present curve of each cytokines was ranging from 9.8 pg/ml to 10000 pg/mL.
Bioactivity-FCM
Please refer to DS document for the assay protocol.
A representative scatter plot showing single bead population in P1 gate (left), the beads conjugated with analyte-sepcific antibody differntiated by APC fluorescence intensity (middle), and fluorescence signal in propotion to concentration of the analyte was measured for each cytokine in PE channel (right). Data was generated on Beckman Cytoflex S Flow Cytometer, equipped with blue laser (488 nm) and red laser (640 nm).
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