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Integrin alpha V beta 3, ITGAV&ITGB3
Human ITGAV&ITGB3 Heterodimer Protein, His Tag&Tag Free (IT3-H52E3) is expressed from human 293 cells (HEK293). It contains AA Phe 31 - Val 992 (ITGAV) & Gly 27 - Asp 718 (ITGB3) (Accession # NP_002201.1 (ITGAV) & NP_000203.2 (ITGB3)).
Predicted N-terminus: Phe 31 (ITGAV) & Gly 27 (ITGB3)
Human ITGAV&ITGB3 Heterodimer Protein, His Tag&Tag Free, produced by co-expression of ITGAV and ITGB3, has a calculated MW of 112.9 kDa (ITGAV) and 81.8 kDa (ITGB3). Subunit ITGAV is fused with an acidic tail at the C-terminus and followed by a polyhistidine tag and subunit ITGB3 contains no tag but a basic tail at the C-terminus. The non-reducing (NR) protein migrates as 135-150 kDa (ITGAV) and 85-90 kDa (ITGB3) respectively due to glycosylation.
Less than 0.01 EU per μg by the LAL method / rFC method.
>95% as determined by SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM NaCl, pH7.5 with trehalose as protectant.
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Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
This product is shipped at ambient temperature.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.