Cell Lines

All of our cell line products are monoclonal cell lines.

According to our current company policy:

• With a signed Non-Disclosure Agreement (NDA), we are authorized to share the vector map.
• Without a signed NDA: We may disclose the sequence of the overexpressed target gene. Additionally, we can provide lentiviral residual testing reports upon request.

The following confidential information is not disclosed:

1. The complete vector sequence and the original vector name.
2. Sequences of signal response elements related to signal transduction pathways.

Cell lines are shipped on dry ice. To ensure optimal cell viability, we recommend thawing and initiating culture immediately upon receipt. If immediate thawing and culturing are not possible, we advise transferring the cells to liquid nitrogen for long-term storage. Please ensure the transfer process is quick to avoid thawing, as this may impact the long-term stability and viability of the cells.

If immediate transfer to liquid nitrogen is not feasible, the cells can be temporarily stored in a -80°C freezer. However, we recommend that the storage period from the date of receipt should not exceed two weeks. Long-term storage on dry ice or in a -80°C freezer is not recommended.

When using the cells, please refer to the recommended thawing and culturing methods provided in the DS.

Please Note: If the cells are received unfrozen or not on dry ice, please contact our technical support team immediately at techsupport@acrobiosystems.com.

We provide two cryovials of cell lines to ensure the smooth progress of your experiments. In the event of any issues with thawing, recovery, or culturing of the first vial, please contact our technical support team (techsupport@acrobiosystems.com) for troubleshooting before thawing the second vial.

All ACRO cell lines undergo pre-shipment validation for recovery and culturing. Additionally, we recommend establishing a cell bank at the earliest possible passage stage to ensure long-term use.

After thawing the cells, they should initially be cultured in a medium without selection antibiotics for 1-2 passages. If the cells exhibit good condition, you can switch to a medium with selection antibiotics for further passaging. For guidance on selecting appropriate antibiotics, please refer to FAQ12.

Additionally, we recommend adding P/S (Penicillin-Streptomycin) to the culture medium throughout the entire cell culture process to maintain aseptic conditions.