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Product Specifications
SourceThis Protein A is expressed from E. coli cells.FormulationLyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.Physical AppearancePowderStorage (unopened)-20°C to -70°CStorage (opened)-70°CUnit Size1000 tests & 5000 tests & 20000 testsTechnologyTR-FRETIntended UseFor binding the Fc fragment of IgG as Acceptor in TR-FRET AssayReconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
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Bioactivity-TR-FRET
Please refer to DS document for the assay protocol.
In this TR-FRET assay, Streptavidin Protein Europium chelate (Cat. No. FRT-TD09) is used as the Donor and Protein A FA-Labeled (Cat. No. FRT-TA10) is used as the Acceptor. Biotinylated Human PD-1 Protein, Avitag,His Tag (Cat. No. PD1-H82E4), at 2 ng/well, can bind to the Monoclonal Anti-Human PD-1 Antibody, Human IgG4 with a linear detection range of 2.5-0.01 μg/mL (QC tested).
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