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Application
N1-Me-pUTP is a common modification in mRNA synthesis. It significantly increases mRNA stability. In the context of residual detection, the standard for dsRNA is designed with N1-Me-pUTP modification. This N1-Me-pUTP dsRNA is specifically intended for use with the Double - Stranded RNA (dsRNA) ELISA Kit (RES - A092). Serving as a standard, it enables the construction of a reliable standard curve. This allows for accurate quantification of dsRNA in samples by comparing absorbance values during the ELISA assay.
Purity
>90% (LC-MS)
Storage
Storage Conditions: store at -20°C to -80°C upon reconstitution. Additional Storage Conditions: avoid freeze/thaw cycles, store in aliquots.
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Length
Double-strand RNA standard was confirmed for purity and length via RNA electrophoresis. The gel was stained with ethidium bromide. The length of dsRNA standard was validated with RNA ladder marker (NEB, N0363S).
Bioactivity-ELISA
N1-Me-pUTP dsRNA (400bp) was detected by Double-Stranded RNA (dsRNA) ELISA Kit (Cat. No. RES-A092), with a linear range of 50-1600 pg/mL.
Protocol
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