ELISA Buffer Set (Phosphate system, 96T)

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EBS-002-96tests
$120.00
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EBS-002
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Materials Provided

ID
Components
Size
EBS002-C01
High-bind plate
1 plate
EBS002-C02
Coating buffer
12 mL
EBS002-C03
10xWashing Buffer
60 mL
EBS002-C04
Blocking buffer
50 mL
EBS002-C05
Substrate solution
12 mL
EBS002-C06
Stop Solution
7 mL

Product Overview

This ELISA Buffer Set-96T contains a High-bind 96well plate, coating buffer, washing buffer, blocking buffer, substrate solution and stop solution. The ELISA Buffer Set-96T can be quickly applied to the ELISA experiment and save your time to prepare buffers. This ELISA Buffer Set-96T is sterile-filtered.

Application

Coating buffer: Use coating buffer to dilute the sample you will coat, add 100μL/well to high-bind plate.

Washing buffer: Dilute the washing buffer to 1x with distilled water before experiment, add 300μL/well for washing the coated plate.

Blocking buffer: Dilute the blocking buffer to 1x with distilled water before experiment, add 300μL/well for blocking the coated plate. The samples and detection antibodies can be prepared by 1x blocking buffer which should be 4 times dilution.

Substrate solution: Add 100μL/well to plate.

Stop solution: Add 50μL/well to plate.

It is for research use only.

Storage

Store this set at 2-8°C, and protect from light. If precipitation occurs, filter to clarify before use.

Assay Principles

This ELISA Buffer Set-96T contains a High-bind 96well plate, coating buffer, washing buffer, blocking buffer, substrate solution and stop solution. The ELISA Buffer Set-96T can be quickly applied to the ELISA experiment and save your time to prepare buffers. This ELISA Buffer Set-96T is sterile-filtered.