Biotinylated FMC63 scFv, Fc,Avitag™

Cat. No. / Size
Price
Availability
Qty
CD9-BV4324b-25ug
$280.00
In Stock - Delivery in 2-3 business days
CD9-BV4324b-200ug
$1420.00
In Stock - Delivery in 2-3 business days

Product Details

  • Source

    Biotinylated FMC63 scFv, Fc, Avitag™ is a recombinant single-chain variable fragment derived from the mouse monoclonal antibody clone FMC63 and expressed in HEK293 cells.

  • Antibody Type

    Recombinant Monoclonal

  • Clone

    FMC63

  • Host Species

    Mouse

  • Reactivity

    Human

  • Immunogen

    CD19

  • Conjugate

    Biotinylated

  • Specificity

    Specifically recognizes the CD19.

  • Application

    Application
    Recommended Use / Performance
    ELISA
    1:5-1:50
  • Purification

    Affinity purification.

  • Aggregation

    Less than 5%, as determined by SEC-MALS.

  • Concentration

    Please refer to the Certificate of Analysis (CoA).

  • Form

    Lyophilized

  • Formulation

    Lyophilized from a 0.22 μm-filtered solution in PBS (pH 7.4), with trehalose as protectant.

    Please contact us for customized product forms or formulations.

  • Reconstitution

    Please refer to the Certificate of Analysis (CoA) for specific instructions.

  • Shipping

    Lyophilized product is shipped at ambient temperature.

  • Storage

    For long term storage, the product should be stored in a lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • Notices

    Product Specific Notices: For research use only.

  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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Performance Data

  • Bioactivity-ELISA

     CD19 ELISA

    A microplate was coated with FMC63 monoclonal antibody, Mouse IgG2a, at 1 μg/mL. Serially diluted Rabbit Anti-Mouse FMC63 Polyclonal Antibody was added, followed by Biotinylated FMC63 scFv-Fc-Avitag at 2 μg/mL. Binding was detected using HRP-conjugated streptavidin. The QC-tested sensitivity was 78 ng/mL (QC tested).

    Protocol

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FAQ

  • product

    Can lyophilized proteins remain stable during room-temperature shipping or temporary room-temperature exposure?

    In general, lyophilized proteins remain stable during room-temperature shipping or temporary exposure to room-temperature conditions.


    Many ACROBiosystems recombinant proteins are supplied in lyophilized (freeze-dried) form. The lyophilization process effectively removes moisture from the product, helping reduce degradation-related reactions and improve protein stability during transportation and storage.


    To evaluate the stability of lyophilized proteins under normal temperature conditions, ACROBiosystems conducted a stability study on 11 representative lyophilized protein products at 37°C. The study demonstrated that these proteins maintained good quality stability after storage at 37°C for approximately 20 days. Based on these validation results, temporary room-temperature exposure during shipping or handling is generally not expected to have a significant impact on product quality or downstream application performance.


    To ensure optimal long-term stability, products should be stored according to the storage conditions specified in the product Certificate of Analysis (COA) upon receipt.


    Supporting Document


Background

FMC63 is an IgG2a mouse monoclonal antibody specific for CD19, which is a target for the immunotherapy of B lineage leukaemias and lymphomas. FMC63 scFv is the most commonly used ectodomain component of CD19-specific CARs. So far, most of reported CART19 trials contain the anti-CD19 scFv derived from FMC63, including the two FDA-approved CARs Kymriah and Yescarta.

Recent Advances

 
Drug Development Progress
  • English Name:

    B-lymphocyte antigen CD19

  • Category:

  • Approved Drugs:

    14 Details

  • Drugs in Clinical Trials:

    435 Details

  • Highest Development Stage:

    Approved

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