Human Ubiquitin Protein, HA Tag (MALS verified)

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Cat. No. / Size
Price
Qty
UBN-H5143-100ug
$350.00
UBN-H5143-500ug
$1320.00
ETA of in-stock products:2 business days

Product Details

  • Synonyms

    HEL-S-50

  • Source

    Human Ubiquitin Protein, HA Tag (UBN-H5143) is expressed from E. coli cells. It contains AA Gln 2 - Gly 76 (Accession # P0CG47-1).

    Predicted N-terminus: Met

    Request for sequence
  • Molecular Characterization

    Ubiquitin Structure

    Other Tags and Version Biotin & Other Labeled Version

    This protein carries HA tag at the N-terminus.

    The protein has a calculated MW of 9.6 kDa. The protein migrates as 8 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE).

  • Purity

    >90% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in 50 mM Tris-HCl, 150 mM NaCl, pH7.5 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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Performance Data

  • SDS-PAGE

    Ubiquitin SDS-PAGE

    Human Ubiquitin Protein, HA Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).

  • SEC-MALS

    Ubiquitin SEC-MALS

    The purity of Human Ubiquitin Protein, HA Tag (Cat. No. UBN-H5143) is more than 90% and the molecular weight of this protein is around 10-16 kDa verified by SEC-MALS.

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Background

Ubiquitin has a major role in targeting cellular proteins for degradation by the 26S proteosome. It is also involved in the maintenance of chromatin structure, the regulation of gene expression, and the stress response. Ubiquitin is synthesized as a precursor protein consisting of either polyubiquitin chains or a single ubiquitin moiety fused to an unrelated protein.

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