제품 수집
제품 세부정보
Synonyms
Triggering receptor expressed on myeloid cells 2, TREM2, TREM-2
Source
Biotinylated Human TREM2, His,Avitag (TR2-H82E7) is expressed from human 293 cells (HEK293). It contains AA His 19 - Ser 174 (Accession # Q9NZC2-1).
Predicted N-terminus: His 19
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).
The protein has a calculated MW of 21.0 kDa. The protein migrates as 29-40 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
Labeling
Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
Protein Ratio
Passed as determined by the HABA assay / binding ELISA.
Purity
>90% as determined by SDS-PAGE.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Shipping and Storage
This product is shipped at ambient temperature.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
ACRO Quality Management System
고객님도 확인한 상품
데이터 표시
SDS-PAGE
Biotinylated Human TREM2, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
Bioactivity-ELISA
Immobilized Biotinylated Human TREM2, His,Avitag (Cat. No. TR2-H82E7) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (2 μg/well) plate can bind Anti-TREM2 Antibody, Human IgG1 with a linear range of 0.2-2 ng/mL (QC tested).
Protocol
Bioactivity-BLI
Loaded Biotinylated Human TREM2, His,Avitag (Cat. No. TR2-H82E7) on SA Biosensor, can bind Human APOE3, His Tag (Cat. No. APE-H5246) with an affinity constant of 37.1 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
Loaded Biotinylated Human TREM2, His,Avitag (Cat. No. TR2-H82E7) on SA Biosensor, can bind Human APOE2 (R154S, R176C) Protein, His Tag (Cat. No. APE-H5256) with an affinity constant of 2.08 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
사용자 리뷰 댓글 남기기
H***o2023-02-15
좋아요We purchased Biotinylated Human TREM2 Protein, His,Avitag™ for Surface Plasmon Resonance (SPR) Analysis of Binding Interactions. The assay worked pretty well with this high-quality product, and we will continue to support ACRO.
배경 소개
프론티어 진행
의약품 개발 진행- 영어 이름：
Triggering receptor expressed on myeloid cells 2
- 카테고리：
- 상장 의약품 수：
0 상세
- 임상 의약품 수：
4 상세
- 최고 개발 단계：
Phase 2 Clinical
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전화
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