Can lyophilized proteins remain stable during room-temperature shipping or temporary room-temperature exposure?

In general, lyophilized proteins remain stable during room-temperature shipping or temporary exposure to room-temperature conditions. Many ACROBiosystems recombinant proteins are supplied in lyophilized (freeze-dried) form. The lyophilization process effectively removes moisture from the product, helping reduce degradation-related reactions and improve protein stability during transportation and storage. To evaluate the stability of lyophilized proteins under normal temperature conditions, ACROBiosystems conducted a stability study on 11 representative lyophilized protein products at 37°C. The study demonstrated that these proteins maintained good quality stability after storage at 37°C for approximately 20 days. Based on these validation results, temporary room-temperature exposure during shipping or handling is generally not expected to have a significant impact on product quality or downstream application performance. To ensure optimal long-term stability, products should be stored according to the storage conditions specified in the product Certificate of Analysis (COA) upon receipt. Supporting Document Validation Report for Accelerated Stability Study of 11 Lyophilized Proteins

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Human TNF-alpha Protein, His Tag, active trimer, low endotoxin (Mass Spec & MALS verified)

Related Products: TR-FRET kit FRT-C02, Cell line CJUR-STF248

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TNA-H5228-100ug

$320.00

In Stock - Delivery in 1-2 business days

TNA-H5228-1mg (250ug X 4)

$1,965.00

In Stock - Delivery in 1-2 business days

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Important Statement
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Synonyms

DIF, TNF-alpha, TNFA, TNFSF2, cachexin, cachectin, TNFα

Source

Human TNF-alpha Protein, His Tag (TNA-H5228) is expressed from human 293 cells (HEK293). It contains AA Val 77 - Leu 233 (Accession # NP_000585.2).

Molecular Characterization

Other Tags and Version Biotin & Other Labeled Version

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 18.3 kDa. The protein migrates as 17-19 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

Mass Spectrometry

Sequence Coverage
Human TNF-alpha Protein, His Tag(TNA-H5228) was digested with trypsin and characterized by LC-MS/MS peptide mapping, achieving 100% sequence coverage (Routinely tested).
N-terminal Sequence Analysis
Val-Arg-Ser-Ser-Ser-Arg-Thr-Pro-Ser-Asp-Lys-Pro-Val-Ala-His
(Routinely tested)

Endotoxin

Less than 0.01 EU per μg by the LAL method / rFC method.

Purity

>95% as determined by SDS-PAGE.

>95% as determined by SEC-MALS.

Formulation

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

Reconstitution

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Shipping and Storage

This product is shipped at ambient temperature.

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.

ACRO Quality Management System

QMS(ISO, GMP)

Quality Advantages

Quality Control Process

배경 소개

Tumor necrosis factor alpha (TNFα) is a cytokine produced primarily by monocytes and macrophages. It is found in synovial cells and macrophages in the tissues.The primary role of TNFα is in the regulation of immune cells. TNFα is able to induce apoptotic cell death, to induce inflammation, and to inhibit tumorigenesis and viral replication. Dysregulation of TNFα production has been implicated in a variety of human diseases, including major depression, Alzheimer's disease and cancer. Recombinant TNFα is used as an immunostimulant under the INN tasonermin. TNFα can be produced ectopically in the setting of malignancy and parallels parathyroid hormone both in causing secondary hypercalcemia and in the cancers with which excessive production is associated.

SDS-PAGE

Human TNF-alpha Protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).

SEC-MALS

The purity of Human TNF-alpha Protein, His Tag (Cat. No. TNA-H5228) is more than 95% and the molecular weight of this protein is around 50-70 kDa verified by SEC-MALS.

Report

Activity-ELISA

Immobilized Human TNF-alpha Protein, His Tag (Cat. No. TNA-H5228) at 0.2 μg/mL (100 μL/well) can bind Human TNFR1, Fc Tag (Cat. No. TN1-H5251) with a linear range of 0.4-13 ng/mL (QC tested).

Protocol

Activity-SPR

Humira (Adalimumab) captured on CM5 chip via anti-human IgG Fc antibodies surface, can bind Human TNF-alpha Protein, His Tag (Cat. No. TNA-H5228) with an affinity constant of 0.244 nM as determined in a SPR assay (Biacore T200) (Routinely tested).

Protocol

Activity-BLI

Loaded Monoclonal Anti-TNF-alpha antibody, Human IgG1 (13B8) (Cat. No. TNA-AM493) on Protein A Biosensor, can bind Human TNF-alpha, His Tag (active trimer) (MALS verified) (Cat. No. TNA-H5228) with an affinity constant of 1.03 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

Protocol

Loaded Monoclonal Anti-TNF-alpha antibody, Human IgG1 (16H5) (Cat. No. TNA-AM494) on Protein A Biosensor, can bind Human TNF-alpha, His Tag (active trimer) (MALS verified) (Cat. No. TNA-H5228) with an affinity constant of 1.72 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

Protocol

Loaded Biotinylated Monoclonal Anti-TNF-alpha Antibody, human IgG1 (16H5) (Cat. No. TNA-BLM494) on Protein A Biosensor, can bind Human TNF-alpha, His Tag (Cat. No. TNA-H5228) with an affinity constant of 2.13 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

Protocol

Activity-CELL BASE

Human TNF-alpha Protein, His Tag (Cat. No. TNA-H5228) induces cytotoxicity effect on the WEH1-13VAR cells in the presence of the metabolic inhibitor actinomycin D. The EC50 for this effect is 0.013-0.074 ng/mL (Routinely tested).

Protocol

Neutralization assay shows that the cytotoxicity effect of Human TNF-alpha Protein, His Tag (Cat. No. TNA-H5228) was inhibited by increasing concentration of Adalimumab. The concentration of TNF-alpha used is 1 ng/mL. The IC50 is 26.8 ng/mL (Routinely tested).

Protocol

인기 검색어:

Human TNF-alpha Protein, His Tag, active trimer, low endotoxin (Mass Spec & MALS verified)

Sequence Coverage

N-terminal Sequence Analysis

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