Product Details
Synonyms
CT120.2, HPP1, TENB2, TPEF, TR, TR-2
Source
Biotinylated Human TMEFF2 Protein, His,Avitag (TM2-H82E3) is expressed from human 293 cells (HEK293). It contains AA Phe 41 - Val 320 (Accession # Q9UIK5-1).
Predicted N-terminus: Phe 41
Request for sequenceMolecular Characterization

Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).
The protein has a calculated MW of 34.5 kDa. The protein migrates as 43-55 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
Labeling
Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
Protein Ratio
Passed as determined by the HABA assay / binding ELISA.
Endotoxin
Less than 1.0 EU per μg by the LAL method / rFC method.
Purity
>90% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 24 months in lyophilized state;
- -70°C for 12 months under sterile conditions after reconstitution.
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Performance Data
SDS-PAGE

Biotinylated Human TMEFF2 Protein, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS

The purity of Biotinylated Human TMEFF2 Protein, His,Avitag (Cat. No. TM2-H82E3) is more than 90% and the molecular weight of this protein is around 40-60 kDa verified by SEC-MALS.
Report
Bioactivity-ELISA

Immobilized Human PDGF-AA Protein, premium grade (Cat. No. PDA-H5113) at 5 μg/mL (100 μL/well) can bind Biotinylated Human TMEFF2 Protein, His,Avitag (Cat. No. TM2-H82E3) with a linear range of 2-39 ng/mL (QC tested).
Protocol
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