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Product Details
Synonyms
TIGIT, VSIG9, VSTM3
Source
PE-Labeled Human TIGIT Protein, His Tag (TIT-HP2H3) is expressed from human 293 cells (HEK293). It contains AA Met 22 - Pro 141 (Accession # Q495A1-1).
Predicted N-terminus: Met 22
Molecular Characterization
This protein carries a polyhistidine tag at the C-terminus.
Conjugate
PE
Excitation Wavelength: 488 nm / 561 nm
Emission Wavelength: 575 nm
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, 0.2% BSA, pH7.4 with trehalose as protectant.
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Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please protect from light and avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Performance Data
Evaluation of CAR expression
FACS Analysis of TIGIT binding to 293T overexpressing CD155 cells
Flow cytometric analysis of 293T overexpressing CD155 cells staining with PE-Labeled Human TIGIT Protein, His Tag (Cat. No. TIT-HP2H3) at 1:50 dilution (2 μL of the stock solution corresponds to labeling of 5e5 cells in a final volume of 100 µL), compared with negative control protein(Fig. B and A). PE signal was used to evaluate the binding activity (QC tested).
Protocol
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Background
Recent Advances
Drug Development Progress- English Name：
T cell immunoglobulin and ITIM domains
- Category：
- Approved Drugs：
0 Details
- Drugs in Clinical Trials：
41 Details
- Highest Development Stage：
Phase 3 Clinical
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