Streptavidin Protein-ALP, Alkaline Phosphatase conjugated Streptavidin

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Cat. No. / Size
Price
Qty
STN-NA117-100ug
$65.00
STN-NA117-1mg
$175.00
ETA of in-stock products:2 business days

Product Details

  • Synonyms

    Streptavidin, SA

  • Source

    Streptavidin is expressed from E. coli cells and conjugated with alkaline phosphatase under optimal conditions.

  • Molecular Characterization

    This protein carries no "tag".

    The protein has a calculated MW of 13.8 kDa.

  • Application

    Recommended for use in ALP Chemiluminescence System such as CLEIA (0.1 µg/mL) or MPCLIA, immunohistochemistry, and western blot applications. Avoid using biotin-containing solutions as diluents. It is recommended that the reagent be titrated for optimal performance for each application. NOTE: Do not use skim milk as a blocking agent in the assay with streptavidin, since skim milk contains free biotin which will cause high backgrounds.

  • Formulation

    Supplied as 0.2 μm filtered solution in 20 mM Tris, 3 M NaCl, pH7.5, 1% BSA, 0.03% Proclin300 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Shipping

    This product is supplied and shipped with dry ice, please inquire the shipping cost.

  • Storage

    Please protect from light and avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. The product MUST be stored at -70°C or lower upon receipt;
    2. -70°C for 3 months under sterile conditions.
  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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Performance Data

  • Bioactivity-CLEIA

     Streptavidin CLEIA

    Immobilized Anti-SARS-CoV-2 Spike RBD Antibody, Chimeric mAb, Human IgG1 (Cat. No. S1N-M12A1) at 1 μg/mL (100 μL/well) can bind Biotinylated SARS-CoV-2 Spike RBD (L452R, T478K), His,Avitag (Cat. No. SPD-C82Ed) with a linear range of 0.0049-10 ng/mL when detected by Streptavidin Protein-ALP, Alkaline Phosphatase conjugated Streptavidin (0.1 µg/mL) (Cat. No. STN-NA117) (QC tested).

    Protocol

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Background

The Streptavidin Protein Europium chelate is a universal tool for TR-FRET assays that can bind biotinylated molecules. This product uses high-purity streptavidin (SA) covalently conjugated with Eu³⁺ chelate, and it can be used in combination with other Acceptors directly or indirectly labeled with fluorescent dyes. When the Donor and Acceptor come into close proximity (within a distance of less than 10 nm), a FRET reaction occurs: the 620 nm signal emitted by the Donor upon excitation by a specific light source is received by the Acceptor, which then emits a 665 nm signal.

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