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SMO, smoothened, frizzled class receptor, CRJS, FZD11, Gx, PHLS, SMOH
Detergent Human SMO Protein, His,Flag Tag (SMO-H52D3) is expressed from human 293 cells (HEK293). It contains AA Arg 28 - Gln 555 (Accession # Q99835-1).
Predicted N-terminus: Arg 28
This protein carries a polyhistidine tag at the C-terminus, followed by a Flag tag. The protein has a calculated MW of 77.0 kDa.
*The DDM/CHS buffer (Cat. No. DC-11) is sold separately and not included in protein, you can follow the link for product information.
Each full-length transmembrane protein is encapsulated by detergent micelle.
Through mild detergent micelle extraction, dedicated solubilization, and stringent affinity purification, we maximally retain the native transmembrane conformation of full-length transmembrane proteins throughout downstream purification. This workflow yields purified, detergent-solubilized proteins free of unrelated membrane impurities and exogenous scaffold proteins, with exceptional purity, accurate quantitation, and well-preserved biological activity—ideal for immunization, binding assays in antibody discovery and screening (ELISA) and affinity testing (SPR and BLI), etc.
Less than 1.0 EU per μg by the LAL method / rFC method.
>90% as determined by SDS-PAGE.
This product is not suitable for cell based experiments due to cytotoxicity of DDM.
DDM and CHS are INDISPENSABLE to keep membrane protein soluble and active, under no circumstances should you remove DDM and CHS.
DDM/CHS buffer (DC-11) is sold separately and not included in protein, and please contact us if you need the buffer.
If glycerol is not compatible with your application, remove glycerol just before the immediate experiment, and NEVER store glycerol-free protein solution.
Supplied as 0.2 μm filtered solution in 50 mM HEPES, 150 mM NaCl, DDM, CHS, pH7.5 with glycerol as protectant.
Contact us for customized product form or formulation.
This product is supplied and shipped on dry ice.
Please avoid repeated freeze-thaw cycles.