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Product Details
Synonyms
CARD3, CARDIAK, CCK, GIG30, RICK, RIP2
Source
Human RIPK2 Protein, GST Tag (RI2-H55G3) is expressed from Baculovirus-Insect cells. It contains AA Met 1 - Glu 299 (Accession # O43353).
Predicted N-terminus: Met 1
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a GST tag at the C-terminus.
The protein has a calculated MW of 59.7 kDa. The protein migrates as 58-65 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin
Less than 1.0 EU per μg by the LAL method / rFC method.
Purity
>80% as determined by SDS-PAGE.
Formulation
Supplied as 0.2 μm filtered solution in 50 mM Tris, 150 mM NaCl, pH7.5 with glycerol as protectant.
Contact us for customized product form or formulation.
Shipping and Storage
This product is supplied and shipped on dry ice.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- The product MUST be stored at -70°C or lower upon receipt;
- -70°C for 3 months under sterile conditions.
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Performance Data
SDS-PAGE
Human RIPK2 Protein, GST Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 80% (With Star Ribbon Pre-stained Protein Marker).
Bioactivity
The RIPK2 assay is performed using the ADP-Glo TM Kinase Assay kit which quantifies the amount of ADP produced by the RIPK2 reaction. The ADP-Glo TM Reagent is added to terminate the kinase reaction and to deplete the remaining ATP, and then the Kinase Detection Reagent is added to convert ADP to ATP and to measure the newly synthesized ATP using luciferase/luciferin reaction. The specific activity is >5 pmol/min/μg (QC tested).
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