Save Product
Product Details
Synonyms
RSPO1, CRISTIN3, FLJ40906, RP11-566C13.1, R-spondin-1
Source
Human R-Spondin 1 (21-146), His Tag, premium grade (RS6-H4220) is expressed from human 293 cells (HEK293). It contains AA Ser 21 - Ala 146 (Accession # Q2MKA7).
Predicted N-terminus: Ser 21
It is produced under our rigorous quality control system that incorporates a comprehensive set of tests including sterility and endotoxin tests. Product performance is carefully validated and tested for compatibility for cell culture use or any other applications in the early preclinical stage. When ready to transition into later clinical phases, we also offer a custom GMP protein service that tailors to your needs. We will work with you to customize and develop a GMP-grade product in accordance with your requests that also meets the requirements for raw and ancillary materials use in cell manufacturing of cell-based therapies.
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 14.6 kDa. The protein migrates as 20-22 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin
Less than 0.1 EU per μg by the LAL method / rFC method.
Sterility
Negative
Mycoplasma
Negative
Purity
>90% as determined by SDS-PAGE.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
ACRO Quality Management System
Customers Also Viewed
Performance Data
SDS-PAGE
Human R-Spondin 1 (21-146), His Tag, premium grade on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
Bioactivity-Organoid Culture
R-spondin-1 (Cat. No. RS6-H4220) maintains intestinal organoid growth well during multiple passages and long-term culture processes, comparable to other companies. The organoids showed good budding morphology.
Human EGF (Cat. No. EGF-H52H3), Noggin (Cat. No. NON-H5257), R-spondin1 (Cat. No. RS6-H4220), FGF7 (Cat. No. FG7-H52H5), FGF10, HGF (Cat. No. HGF-H52H3) actively support liver ductal organoid growth.
iPSC derived intestinal organoids forming cryptic structure were cultured with R-Spondin 1 (Cat. No. RS6-H4220).
Bioactivity-CELL BASE
Human R-Spondin 1 (21-146), His Tag, premium grade (Cat. No. RS6-H4220) stimulates TCF/LEF (Luc) HEK293 Reporter Cell. The specific activity of Human R-Spondin 1 (21-146), His Tag, premium grade is >1.00 X 10^4 U/mg (QC tested).
Protocol
Bioactivity-ELISA
Immobilized Human R-Spondin 1 (21-146), His Tag, premium grade (Cat. No. RS6-H4220) at 5 μg/mL (100 μL/well) can bind Human RNF43, Fc Tag (Cat. No. RN3-H5256) with a linear range of 0.01-0.156 μg/mL (QC tested).
Protocol
Immobilized Human R-Spondin 1 (21-146), His Tag, premium grade (Cat. No. RS6-H4220) at 5 μg/mL (100 μL/well) can bind Human LRP-6 (20-630), Mouse IgG2a Fc Tag (Cat. No. LR6-H5253) with a linear range of 0.039-2.5 μg/mL (Routinely tested).
Protocol
Customer Reviews Writing Reviews
Background
Recent Advances
Contact Us
전화
글로벌: +1 800-810-0816 (무료)
아시아태평양: +86 400-682-2521
Drug Candidate Licensing
