Influenza A virus (strain A/Victoria/3/1975 H3N2) Polymerase acidic Protein, His Tag

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PON-V51H3-100ug
$380.00
In Stock - Delivery in 2-3 business days
PON-V51H3-1mg
$2800.00
In Stock - Delivery in 2-3 weeks
총 항목 수 제품 금액$ 0

제품 세부정보

  • Synonyms

    Polymerase acidic protein

  • Source

    Influenza A virus (strain A/Victoria/3/1975 H3N2) Polymerase acidic Protein, His Tag (PON-V51H3) is expressed from E. coli cells. It contains AA Met 1 - Gly 209 (Accession # P31343-1).

    Predicted N-terminus: Met 1

    Request for sequence
  • Molecular Characterization

    Polymerase acidic protein Structure

    Other Tags and Version Biotin & Other Labeled Version

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 26.5 kDa. The protein migrates as 28-30 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under non-reducing (NR) condition (SDS-PAGE).

  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Supplied as 0.2 μm filtered solution in PBS, 0.5 M Arginine, Glycerol, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Shipping and Storage

    This product is supplied and shipped on dry ice.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. The product MUST be stored at -70°C or lower upon receipt;
    2. -70°C for 3 months under sterile conditions.
  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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데이터 표시

  • SDS-PAGE

    Polymerase acidic protein SDS-PAGE

    Influenza A virus (strain A/Victoria/3/1975 H3N2) Polymerase acidic Protein, His Tag on SDS-PAGE under non-reducing (NR) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).

  • Bioactivity-ELISA

     Polymerase acidic protein ELISA

    Immobilized Influenza A virus (strain A/Victoria/3/1975 H3N2) Polymerase acidic Protein, His Tag (Cat. No. PON-V51H3) at 1 μg/mL (100 μL/well) can bind Influenza A PA Polyclonal Antibody,Rabbit/IgG with a linear range of 0.2-16 ng/mL (QC tested).

    Protocol

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배경 소개

Plays an essential role in viral RNA transcription and replication by forming the heterotrimeric polymerase complex together with PB1 and PB2 subunits. The complex transcribes viral mRNAs by using a unique mechanism called cap-snatching. It consists in the hijacking and cleavage of host capped pre-mRNAs. These short capped RNAs are then used as primers for viral mRNAs. The PB2 subunit is responsible for the binding of the 5' cap of cellular pre-mRNAs which are subsequently cleaved after 10-13 nucleotides by the PA subunit that carries the endonuclease activity.

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