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Human Perlecan Protein, His Tag, low endotoxin

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PEN-H5244-100ug
$350.00
In Stock - Delivery in 1-2 business days
PEN-H5244-1mg
$2,400.00
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PEN-H5244
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Product Details
Performance Data
Reviews & Citations
Resources
  • Product Details
  • Performance Data
  • Reviews & Citations
  • Important Statement
  • Resources
Synonyms

Perlecan

Source

Human Perlecan Protein, His Tag (PEN-H5244) is expressed from human 293 cells (HEK293). It contains AA Val 3295 - Ile 3394 (Accession # P98160).

Predicted N-terminus: His

Molecular Characterization
Other Tags and Version Biotin & Other Labeled Version

This protein carries a polyhistidine tag at the N-terminus.

The protein has a calculated MW of 12.7 kDa. The protein migrates as 13-14 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

Endotoxin

Less than 0.01 EU per μg by the LAL method / rFC method.

Purity

>90% as determined by SDS-PAGE.

Formulation

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

Reconstitution

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Shipping and Storage

This product is shipped at ambient temperature.

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:
  • -20°C to -70°C for 12 months in lyophilized state;
  • -70°C for 3 months under sterile conditions after reconstitution.
ACRO Quality Management System

Background

Perlecan (HSPG2) is an exceptionally large (4391 amino acids, 468 kDa) heparan sulfate proteoglycan encoded by the HSPG2 gene. Characterized by its string of pearls appearance and five distinct domains (I-V), Perlecan is a ubiquitous and essential component of all native basement membranes and the extracellular matrix (ECM). It is synthesized by various cell types, including vascular endothelial and smooth muscle cells, and plays a critical role in binding and cross-linking numerous ECM components and cell-surface molecules, thereby contributing to tissue architecture and mediating cell-matrix interactions.