제품 세부정보
Synonyms
PDCD1, PD1, CD279, SLEB2
Source
MABSol® Biotinylated Mouse PD-1, Fc,Avitag (PD1-M82F4) is expressed from human HEK293 cells. It contains AA Leu 25 - Gln 167 (Accession # NP_032824).
Predicted N-terminus: Leu 25
Request for sequenceMolecular Characterization

Other Tags and Version Biotin & Other Labeled Version
This protein carries a human IgG1 Fc tag at the C-terminus, followed by an Avi tag (Avitag™).
The protein has a calculated MW of 44.6 kDa. The protein migrates as 60-66 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Labeling
Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
Protein Ratio
Passed as determined by the HABA assay / binding ELISA.
Purity
>90% as determined by SDS-PAGE.
>95% as determined by SEC-MALS.
Formulation
Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Shipping and Storage
This product is shipped at ambient temperature.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
ACRO Quality Management System
고객님도 확인한 상품
데이터 표시
SDS-PAGE

Biotinylated Mouse PD-1, Fc,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.
SEC-MALS

The purity of Biotinylated Mouse PD-1, Fc,Avitag (Cat. No. PD1-M82F4) is more than 95% and the molecular weight of this protein is around 112-136 kDa verified by SEC-MALS.
Report
Bioactivity-ELISA

Immobilized Mouse PD-L1, Fc Tag (Cat. No. PD1-M5251) at 10 μg/mL (100 μL/well) can bind Biotinylated Mouse PD-1, Fc,Avitag (Cat. No. PD1-M82F4) with a linear range of 0.08-2.5 μg/mL (QC tested).
Protocol
Bioactivity-BLI

Loaded Biotinylated Mouse PD-1, Fc,Avitag (Cat. No. PD1-M82F4) on SA Biosensor, can bind Mouse PD-L1 / B7-H1 Protein, His Tag (Cat. No. PD1-M5220) with an affinity constant of 3.8 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
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배경 소개
프론티어 진행
- 영어 이름:
Programmed cell death protein 1
- 카테고리:
- 상장 의약품 수:
22 상세
- 임상 의약품 수:
193 상세
- 최고 개발 단계:
Approved
전화
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