Human PADI4 Protein, His Tag (active enzyme, MALS verified)

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제품 번호/규격
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PA4-H55H3-50ug
$715.00
PA4-H55H3-1mg
$5190.00
ETA of in-stock products:2 business days
총 항목 수 제품 금액$ 0

제품 세부정보

  • Synonyms

    Protein-arginine deiminase type-4, HL-60 PAD, Peptidylarginine deiminase IV, Protein-arginine deiminase type IV, PADI4, EC:3.5.3.15

  • Source

    Human PADI4 Protein, His Tag (PA4-H55H3) is expressed from Baculovirus-Insect cells. It contains AA Met 1 - Pro 663 (Accession # Q9UM07-1).

    Predicted N-terminus: Met 1

    Request for sequence
  • Molecular Characterization

    PADI4 Structure

    Other Tags and Version Biotin & Other Labeled Version

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 76.0 kDa. The protein migrates as 70-85 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

    The protein is designed as a dimer.

  • Endotoxin

    Less than 1.0 EU per μg by the LAL method / rFC method.

  • Purity

    >90% as determined by SDS-PAGE.

    >95% as determined by SEC-MALS.

  • Formulation

    Supplied as 0.2 μm filtered solution in 20 mM Tris, 200 mM NaCl, pH8.0 with glycerol as protectant.

    Contact us for customized product form or formulation.

  • Shipping and Storage

    This product is supplied and shipped on dry ice.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. The product MUST be stored at -70°C or lower upon receipt;
    2. -70°C for 3 months under sterile conditions.
  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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데이터 표시

  • SDS-PAGE

    PADI4 SDS-PAGE

    Human PADI4 Protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).

  • SEC-MALS

    PADI4 SEC-MALS

    The purity of Human PADI4 Protein, His Tag (Cat. No. PA4-H55H3) is more than 95% and the molecular weight of this protein is around 150-180 kDa verified by SEC-MALS.

    Report
  • Bioactivity

    Measured by its ability to catalyze the deimination of benzoyl-arginine ethyl ester. The specific activity is >1000 pmol/min/μg (QC tested).

    Protocol

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배경 소개

Protein arginine deiminase, type 4 (PADI4) is a calcium dependent enzyme Catalyzes the citrullination/deimination of arginine residues of proteins such as histones, thereby playing a key role in histone code and regulation of stem cell maintenance. PADI4 can interact with tumor suppressor p53 and regulate the transcriptional activity of p53. Dysregulation of PADI4 is implicated in a variety of diseases, including rheumatoid arthritis, tumor development, and multiple sclerosis.

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