Human PADI2 Protein, His Tag, low Endotoxin (InsectCells) (active enzyme)

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제품 번호/규격
가격
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PA2-H5543-50ug
$410.00
PA2-H5543-1mg
$3170.00
ETA of in-stock products:2 business days
총 항목 수 제품 금액$ 0

제품 세부정보

  • Synonyms

    PADI2, PAD-H19, KIAA0994, PAD2, PDI2

  • Source

    Human PADI2, His Tag (PA2-H5543) is expressed from Baculovirus-Insect cells. It contains AA Met 1 - Pro 665 (Accession # Q9Y2J8-1).

    Predicted N-terminus: Met

    Request for sequence
  • Molecular Characterization

    PADI2 Structure

    Other Tags and Version Biotin & Other Labeled Version

    This protein carries a polyhistidine tag at the N-terminus.

    The protein has a calculated MW of 77.6 kDa. The protein migrates as 66-70 kDa under reducing (R) condition (SDS-PAGE).

  • Endotoxin

    Less than 0.01 EU per μg by the LAL method / rFC method.

  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Supplied as 0.2 μm filtered solution in PBS, pH7.4 with glycerol as protectant.

    Contact us for customized product form or formulation.

  • Shipping and Storage

    This product is supplied and shipped on dry ice.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. The product MUST be stored at -70°C or lower upon receipt;
    2. -70°C for 3 months under sterile conditions.
  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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데이터 표시

  • SDS-PAGE

    PADI2 SDS-PAGE

    Human PADI2, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

  • Bioactivity-ELISA

     PADI2 ELISA

    Immobilized Human PADI2, His Tag (Cat. No. PA2-H5543) at 1 μg/mL (100 μL/well) can bind Human PADI2 Antibody-Polyclonal Sheep IgG with a linear range of 0.004-1 μg/mL (QC tested).

    Protocol
  • Bioactivity

    Measured by its ability to catalyze the deamination of benzoyl-arginine ethyl ester. The specific activity is >1200 pmol/min/μg (QC tested).

    Protocol

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배경 소개

Protein-arginine deiminase type-2 (PADI2) are calcium-dependent histone-modifying enzymes whose activity is dysregulated in inflammatory diseases and cancer. This enzyme which catalyze the post-translational deimination of proteins by converting arginine residues into citrullines in the presence of calcium ions. The type II enzyme is the most widely expressed family member. Known substrates for this enzyme include myelin basic protein in the central nervous system and vimentin in skeletal muscle and macrophages.

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