Mouse NOTCH1 Protein, His Tag

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제품 번호/규격
가격
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NO1-M52H8-100ug
$360.00
NO1-M52H8-1mg
$2615.00
ETA of in-stock products:2 business days
총 항목 수 제품 금액$ 0

제품 세부정보

  • Synonyms

    NOTCH1, Notch 1, hN1, TAN1

  • Source

    Mouse NOTCH1, His Tag (NO1-M52H8) is expressed from human 293 cells (HEK293). It contains AA Ala 19 - Gln 526 (Accession # Q01705-1).

    Predicted N-terminus: Ala 19

    Request for sequence
  • Molecular Characterization

    NOTCH1 Structure

    Other Tags and Version Biotin & Other Labeled Version

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 55.4 kDa. The protein migrates as 58-68 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin

    Less than 1.0 EU per μg by the LAL method / rFC method.

  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Shipping and Storage

    This product is shipped at ambient temperature.

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

고객님도 확인한 상품

데이터 표시

  • SDS-PAGE

    NOTCH1 SDS-PAGE

    Mouse NOTCH1, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

  • Bioactivity-ELISA

     NOTCH1 ELISA

    Immobilized Human DLL4 Protein, Fc Tag (Cat. No. DL4-H5259) at 5 μg/mL (100 μL/well) can bind Mouse NOTCH1, His Tag (Cat. No. NO1-M52H8) with a linear range of 0.02-0.313 μg/mL (QC tested).

    Protocol

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배경 소개

NOTCH1 Interacts with DNER, DTX1, DTX2 and RBPJ/RBPSUH. Also interacts with MAML1, MAML2 and MAML3 which act as transcriptional coactivators for NOTCH1. The NOTCH1 intracellular domain interacts with SNW1; the interaction involves multimerized NOTCH1 NICD and is implicated in a formation of an intermediate preactivation complex which associates with DNA-bound CBF-1/RBPJ. The activated membrane-bound form interacts with AAK1 which promotes NOTCH1 stabilization. Functions as a receptor for membrane-bound ligands Jagged-1 (JAG1), Jagged-2 (JAG2) and Delta-1 (DLL1) to regulate cell-fate determination. Involved in the maturation of both CD4+ and CD8+ cells in the thymus. Important for follicular differentiation and possibly cell fate selection within the follicle. During cerebellar development, functions as a receptor for neuronal DNER and is involved in the differentiation of Bergmann glia.

프론티어 진행

 
의약품 개발 진행
  • 영어 이름:

    Neurogenic locus notch homolog protein 1

  • 카테고리:

  • 상장 의약품 수:

    0 상세

  • 임상 의약품 수:

    1 상세

  • 최고 개발 단계:

    Phase 1 Clinical

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