Biotinylated Human Neuropilin-2 / NRP2 Protein, His,Avitag™ (MALS verified)

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제품 번호/규격
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NR2-H82E3-25ug
$368.00
NR2-H82E3-200ug
$1449.00
ETA of in-stock products:2 business days
총 항목 수 제품 금액$ 0

제품 세부정보

  • Synonyms

    Neuropilin-2, NRP2, VEGF165R2, Neuropilin2, Vascular endothelial cell growth factor 165 receptor 2

  • Source

    Biotinylated Human Neuropilin-2 Protein, His,Avitag (NR2-H82E3) is expressed from human 293 cells (HEK293). It contains AA Arg 21 - Pro 864 (Accession # O60462-1).

    Predicted N-terminus: Arg 21

    Request for sequence
  • Molecular Characterization

    Neuropilin-2 Structure

    Other Tags and Version Biotin & Other Labeled Version

    This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).

    The protein has a calculated MW of 98.7 kDa. The protein migrates as 130-150 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Labeling

    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

  • Protein Ratio

    Passed as determined by the HABA assay / binding ELISA.

  • Purity

    >90% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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데이터 표시

  • SDS-PAGE

    Neuropilin-2 SDS-PAGE

    Biotinylated Human Neuropilin-2 Protein, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).

  • SEC-MALS

    Neuropilin-2 SEC-MALS

    The purity of Biotinylated Human Neuropilin-2 Protein, His,Avitag (Cat. No. NR2-H82E3) is more than 90% and the molecular weight of this protein is around 100-150 kDa verified by SEC-MALS.

    Report
  • Bioactivity-ELISA

     Neuropilin-2 ELISA

    Immobilized Human VEGF165, premium grade (Cat. No. VE5-H4210) at 5 μg/mL (100 μL/well) can bind Biotinylated Human Neuropilin-2 Protein, His,Avitag (Cat. No. NR2-H82E3) with a linear range of 0.08-5 μg/mL (QC tested).

    Protocol

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배경 소개

Neuropilin-1 (Npn-1, previously known as neuropilin) and Neuropilin-2 (previously known as Npn-1-related molecule) are type I transmembrane proteins that bind distinct members of the class III secreted semaphorin subfamily that are implicated in repulsive axon guidance. Neuropilin extracellular domains contain two CUB (complement-binding) domains, two domains with homology to coagulation factors V and VIII, and a MAM (meprin) domain. In the absence of ligands, neuropilins can form homo- and hetero-oligomers via their MAM domains. Expression in developing neurons of the central and peripheral nervous systems is somewhat overlapping but distinct. Npn-1 and Npn-2 are also receptors for VEGF165 on endothelial and tumor cells.

프론티어 진행

 
의약품 개발 진행
  • 영어 이름:

    Neuropilin 2

  • 카테고리:

  • 상장 의약품 수:

    0 상세

  • 임상 의약품 수:

    1 상세

  • 최고 개발 단계:

    Phase 3 Clinical

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