제품 세부정보
Synonyms
NA, Neuraminidase
Source
Influenza A virus (A/Thailand/8/2022) NA(H3N2) Protein, His Tag (NEE-V524w) is expressed from human 293 cells (HEK293). It contains AA Ile 30 - Thr 469 (Accession # EPI2178976, GISAID).
Molecular Characterization
This protein carries a polyhistidine tag at the N-terminus.
The protein has a calculated MW of 57.0 kDa. The protein migrates as 80-95 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin
Less than 1.0 EU per μg by the LAL method / rFC method.
Purity
>90% as determined by SDS-PAGE.
Formulation
Supplied as 0.2 μm filtered solution in 50 mM Tris, 500 mM NaCl, pH7.5 with trehalose as protectant.
Contact us for customized product form or formulation.
Shipping and Storage
This product is supplied and shipped on dry ice.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- The product MUST be stored at -70°C or lower upon receipt;
- -70°C for 3 months under sterile conditions.
ACRO Quality Management System
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데이터 표시
SDS-PAGE

Influenza A virus (A/Thailand/8/2022) NA(H3N2) Protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
Bioactivity-ELISA

Immobilized Influenza A virus (A/Thailand/8/2022) NA(H3N2) Protein, His Tag (Cat. No. NEE-V524w) at 5 μg/mL (100 μL/well) can bind Anti-NA(A/PAN), Mouse IgG with a linear range of 0.078-2.5 μg/mL (Routinely tested).
Protocol
Bioactivity-SPR

a-Neu5Ac-PAA-biotin immobilized on SA Chip can bind Influenza A virus (A/Thailand/8/2022) NA(H3N2) Protein, His Tag (Cat. No. NEE-V524w) with an affinity constant of 94.1 nM as determined in a SPR assay (Biacore 8K) (QC tested).
Protocol
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