제품 수집
제품 세부정보
Synonyms
NECTIN4, LNIR, PRR4, PVRL4
Source
Biotinylated Human Nectin-4 (32-153), His,Avitag (NE4-H82Ea) is expressed from human 293 cells (HEK293). It contains AA Gly 32 - Leu 153 (Accession # Q96NY8-1).
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the N-terminus, followed by an Avi tag (Avitag™).
The protein has a calculated MW of 16.9 kDa. The protein migrates as 18-20 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Labeling
Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
Protein Ratio
Passed as determined by the HABA assay / binding ELISA.
Purity
>95% as determined by SDS-PAGE.
>95% as determined by SEC-MALS.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Shipping and Storage
This product is shipped at ambient temperature.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
ACRO Quality Management System
고객님도 확인한 상품
데이터 표시
SDS-PAGE
Biotinylated Human Nectin-4 (32-153), His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
SEC-MALS
The purity of Biotinylated Human Nectin-4 (32-153), His,Avitag (Cat. No. NE4-H82Ea) is more than 95% and the molecular weight of this protein is around 16-24 kDa verified by SEC-MALS.
Report
Bioactivity-ELISA
Immobilized Biotinylated Human Nectin-4 (32-153), His,Avitag (Cat. No. NE4-H82Ea) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Anti-Nectin-4 Antibody, Mouse IgG1 with a linear range of 1-4 ng/mL (QC tested).
Protocol
사용자 리뷰 댓글 남기기
O***s2022-07-30
좋아요We used this for an important reagent in discovery campaigns. This was a great product! It came sooner than expected, which was awesome because we were able to easily make deadlines! It was also high quality. We didn't have any problems with it, so I am very happy with the quality of service and product. I will definitely buy again. Thank you very much.
배경 소개
프론티어 진행
의약품 개발 진행- 영어 이름：
Nectin-4
- 카테고리：
- 상장 의약품 수：
1 상세
- 임상 의약품 수：
28 상세
- 최고 개발 단계：
Approved
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전화
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