제품 수집
제품 세부정보
Synonyms
LILRB2, ILT4, LIR2, MIR10, CD85d
Source
Human LILRB2, Fc Tag (LI2-H5253) is expressed from human 293 cells (HEK293). It contains AA Gln 22 - Val 461 (Accession # AAH36827.1).
Predicted N-terminus: Gln 22
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a human IgG1 Fc tag at the C-terminus.
The protein has a calculated MW of 74.2 kDa. The protein migrates as 95 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE).
Endotoxin
Less than 1.0 EU per μg by the LAL method / rFC method.
Purity
>90% as determined by SDS-PAGE.
>95% as determined by SEC-MALS.
Formulation
Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, 25 mM Arginine, 150 mM NaCl, pH7.5 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Shipping and Storage
This product is shipped at ambient temperature.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
ACRO Quality Management System
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데이터 표시
SDS-PAGE
Human LILRB2, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Human LILRB2, Fc Tag (Cat. No. LI2-H5253) is more than 95% and the molecular weight of this protein is around 160-190 kDa verified by SEC-MALS.
Report
Bioactivity-ELISA
Immobilized Human LILRB2, Fc Tag (Cat. No. LI2-H5253) at 1 μg/mL (100 μL/well) can bind Anti-LILRB2 Antibody, Human IgG1 with a linear range of 0.2-2 ng/mL (QC tested).
Protocol
Serial dilutions of Monoclonal Anti-LILRB2 Antibody, Human IgG1 were added into Human LILRB2, Fc Tag (Cat. No. LI2-H5253) : Biotinylated Human HLA-G&B2M&Peptide (RIIPRHLQL) Tetramer Protein, His,Avitag (Cat. No. HLG-H52E9) binding reactions. The half maximal inhibitory concentration (IC50) is 0.70 μg/mL (Routinely tested).
Protocol
Immobilized Biotinylated Human HLA-G&B2M&Peptide (RIIPRHLQL) Complex Protein (Cat. No. HLM-H82E4) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Human LILRB2, Fc Tag (Cat. No. LI2-H5253) with a linear range of 0.6-10 ng/mL (Routinely tested).
Protocol
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배경 소개
프론티어 진행
의약품 개발 진행- 영어 이름：
Leukocyte immunoglobulin-like receptor subfamily B member 2
- 카테고리：
- 상장 의약품 수：
0 상세
- 임상 의약품 수：
11 상세
- 최고 개발 단계：
Phase 2 Clinical
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