Human Laminin 521 Protein, Research Grade (Flagship)Research use only

  • Empower Stem Cell Culture: Basement Membrane Extract Alternative at No Extra Cost!
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Cat. No. / Size
Price
Qty
LA5-H5246-100ug
$40.00
LA5-H5246-1mg (500ug X 2)
$210.00
ETA of in-stock products:2 business days

Product Details

  • The schematic diagram of Laminin in ES/iPS cell culture

  • Product Details

    Laminin 521 offers a defined surface for feeder-free culture of human pluripotent stem cells (PSCs). It maintains normal growth and stemness in ESC, iPSC, and MSC lines. Laminin 521 supports PSC growth for over 10 passages without karyotypic abnormalities and retains differentiation ability into all three germ layers.
    This product is specifically designed for academic research applications and is an alternative to basement membrane extract at No Extra Cost.
    This product is designed and developed utilizing our proprietary AI technology, which is protected by patent rights. We retain full commercial rights to this product.

    RG Laminin 521 vs. Basement Membrane Extract
    Defined matrix and good lot-to-lot consistency;
    Easy to handle (not to handle in 4℃);
    Support homogenous monolayer and faster growth;
    Avoid cell differentiation;
    Cost-effective, comparable pricing to Basement Membrane Extract or Vitronectin.

    RG Laminin 521 vs. Vitronectin
    Better cell survival in colony formation;
    Better cell adhesion ability during cardiac cell differentiation.

  • Features and Advantages

    1. Native Conformation: Native sequences, tag-free and natural function.
    2. Reliable Activity: Biological activity calibrated against WHO/NIBSC standards.
    3. Stringent Quality Control: Protein content, purity, and cell-based bioactivity testing for each batch.
    4. Lowest Endotoxin level (<0.01 EU/ug).
    5. Safety Assurance: Sterile filtration through 0.2 μm membrane.
    6. AOF: Animal origin-free raw materials throughout the production process.
  • Expression System

    HEK293

  • Key Parameter

    Purity (SDS PAGE)
    > 90%
    Endotoxin Test
    <0.5 EU per μg
  • Formulation

    Supplied as 0.2 μm filtered solution in PBS, Glycerol, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Shipping

    This product is supplied and shipped with dry ice, please inquire the shipping cost.

  • Storage

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -70°C for 3 years under sterile conditions;
    2. 2-8°C for 3 months under sterile conditions.
  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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Performance Data

  • Bioactivity-SPR

     Laminin 521 SPR

    Human Laminin 521 Protein, Research Grade (Flagship) (Cat. No. LA5-H5246) immobilized on CM5 Chip can bind Human ITGA6&ITGB1 Heterodimer Protein, His Tag&Tag Free (Cat. No. IT1-H52W7) with an affinity constant of 1.09 nM as determined in a SPR assay (Biacore 8K) (QC tested).

    Protocol

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