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Integrin alpha V beta 6, ITGAV&ITGB6
Rat ITGAV&ITGB6 Heterodimer, His Tag&Tag Free (IT6-R52E6) is expressed from human 293 cells (HEK293). It contains AA Phe 31 - Pro 988 (ITGAV) & Gly 22 - Pro 708 (ITGB6) (Accession # NP_001385621.1 (ITGAV) & Q6AYF4 (ITGB6)).
Predicted N-terminus: Phe 31 & Gly 22
Rat ITGAV&ITGB6 Heterodimer, His Tag&Tag Free, produced by co-expression of ITGAV and ITGB6, has a calculated MW of 112.3 kDa (ITGAV) & 80.1 kDa (ITGB6). Subunit ITGAV is fused with an acidic tail at the C-terminus and followed by a polyhistidine tag and subunit ITGB6 contains no tag but a basic tail at the C-terminus. The protein migrates as 125-145 kDa (ITGAV) and 70-85 kDa (ITGB6) when calibrated against Star Ribbon Pre-stained Protein Marker under non-reducing (NR) condition (SDS-PAGE) due to glycosylation.
Less than 1.0 EU per μg by the LAL method / rFC method.
>90% as determined by SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM NaCl, pH7.5 with trehalose as protectant.
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Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
This product is shipped at ambient temperature.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.