Mouse Integrin alpha V beta 3 (ITGAV&ITGB3) Heterodimer Protein, His Tag&Tag Free (MALS verified)

Synonyms

Integrin alpha V beta 3, ITGAV&ITGB3

Source

Mouse ITGAV&ITGB3 Heterodimer Protein, His Tag&Tag Free (IT3-M52W3) is expressed from human 293 cells (HEK293). It contains AA Phe 31 - Val 988 | Glu 26 - Asp 717 (Accession # P43406-1 | O54890).

Predicted N-terminus: Phe 31 | Glu 26

Molecular Characterization

Other Tags and Version Biotin & Other Labeled Version

The protein has a calculated MW of 112.5 kDa | 81.6 kDa. The protein migrates as 95-105 kDa, 120-130 kDa and 140-160 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

The protein is designed as a heterodimer.

Endotoxin

Less than 1.0 EU per μg by the LAL method / rFC method.

Purity

>95% as determined by SDS-PAGE.

>90% as determined by SEC-MALS.

Formulation

Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM NaCl, pH7.5 with trehalose as protectant.

Contact us for customized product form or formulation.

Reconstitution

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Shipping and Storage

This product is shipped at ambient temperature.

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

1. -20°C to -70°C for 12 months in lyophilized state;
2. -70°C for 3 months under sterile conditions after reconstitution.

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