제품 수집
제품 세부정보
Synonyms
Integrin alpha V beta 3, ITGAV&ITGB3
Source
Biotinylated Human ITGAV&ITGB3 Heterodimer Protein, His,Avitag&Tag Free (IT3-H82W9) is expressed from human 293 cells (HEK293). It contains AA Phe 31 - Val 992 (ITGAV) & Gly 27 - Asp 718 (ITGB3) (Accession # P06756-1(V783I) & P05106-1).
Predicted N-terminus: Phe 31 (ITGAV) & Gly 27 (ITGB3)
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
Biotinylated Human ITGAV&ITGB3 Heterodimer Protein, His, Avitag™&Tag Free, produced by co-expression of ITGAV and ITGB3, has a calculated MW of 114.6 kDa (ITGAV) and 81.8 kDa (ITGB3). Subunit ITGAV is fused with an acidic tail at the C-terminus and followed by a polyhistidine tag and an Avi tag (Avitag™) and subunit ITGB3 contains no tag but a basic tail at the C-terminus. The predicted N-terminus is Phe 31 (ITGAV) & Gly 27 (ITGB3). The non-reducing (NR) protein migrates as 135 kDa (ITGAV) & 80 kDa (ITGB3) respectively due to glycosylation.
Labeling
Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
Protein Ratio
Passed as determined by the SABA assay / HABA assay / binding ELISA.
Purity
>95% as determined by SDS-PAGE.
Formulation
Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM NaCl, pH7.5 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Shipping and Storage
This product is shipped at ambient temperature.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
ACRO Quality Management System
고객님도 확인한 상품
데이터 표시
SDS-PAGE
Biotinylated Human ITGAV&ITGB3 Heterodimer Protein, His,Avitag&Tag Free on SDS-PAGE under non-reducing (NR) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
SEC-MALS
The purity of Biotinylated Human ITGAV&ITGB3 Heterodimer Protein, His,Avitag&Tag Free (Cat. No. IT3-H82W9) is more than 85% and the molecular weight of this protein is around 200-245 kDa verified by SEC-MALS.
Report
Bioactivity-ELISA
Immobilized Human Vitronectin Protein, premium grade (Cat. No. VIN-H5214) at 5 μg/mL (100 μL/well) can bind Biotinylated Human ITGAV&ITGB3 Heterodimer Protein, His,Avitag&Tag Free (Cat. No. IT3-H82W9) with a linear range of 0.01-1.25 μg/mL (QC tested).
Protocol
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FAQ
- product
Can lyophilized proteins remain stable during room-temperature shipping or temporary room-temperature exposure?
In general, lyophilized proteins remain stable during room-temperature shipping or temporary exposure to room-temperature conditions.
Many ACROBiosystems recombinant proteins are supplied in lyophilized (freeze-dried) form. The lyophilization process effectively removes moisture from the product, helping reduce degradation-related reactions and improve protein stability during transportation and storage.
To evaluate the stability of lyophilized proteins under normal temperature conditions, ACROBiosystems conducted a stability study on 11 representative lyophilized protein products at 37°C. The study demonstrated that these proteins maintained good quality stability after storage at 37°C for approximately 20 days. Based on these validation results, temporary room-temperature exposure during shipping or handling is generally not expected to have a significant impact on product quality or downstream application performance.
To ensure optimal long-term stability, products should be stored according to the storage conditions specified in the product Certificate of Analysis (COA) upon receipt.
Supporting Document
배경 소개
프론티어 진행
의약품 개발 진행- 영어 이름：
Integrin alpha-V/beta-3
- 카테고리：
- 상장 의약품 수：
0 상세
- 임상 의약품 수：
31 상세
- 최고 개발 단계：
NDA/BLA
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