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Synonyms
Integrin alpha 6 beta 4, ITGA6&ITGB4
Source
Cynomolgus ITGA6&ITGB4 Heterodimer Protein, His Tag&Tag Free (IN4-C52W3) is expressed from human 293 cells (HEK293). It contains AA Phe 24 - Gly 1012 (ITGA6) & Asn 28 - Ser 711 (ITGB4) (Accession # A0A2K5WLQ4 (ITGA6) & A0A2K5TWI7 (ITGB4)).
Predicted N-terminus: Phe 24 (ITGA6) & Asn 28 (ITGB4)
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
Cynomolgus ITGA6&ITGB4 Heterodimer Protein, His Tag&Tag Free, produced by co-expression of ITGA6 and ITGB4, has a calculated MW of 116.8 kDa (ITGA6) & 82 kDa (ITGB4). Subunit ITGA6 is fused with flag tag at the C-terminus and subunit ITGB4 contains no tag. The protein migrates as 110-130 kDa (ITGA6) and 90-95 kDa (ITGB4) when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
The protein is designed as a heterodimer.
Endotoxin
Less than 1.0 EU per μg by the LAL method / rFC method.
Purity
>90% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Formulation
Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM NaCl, 0.2 M Arginine, pH7.5 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Shipping and Storage
This product is shipped at ambient temperature.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
ACRO Quality Management System
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SDS-PAGE
Cynomolgus ITGA6&ITGB4 Heterodimer Protein, His Tag&Tag Free on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Cynomolgus ITGA6&ITGB4 Heterodimer Protein, His Tag&Tag Free (Cat. No. IN4-C52W3) is more than 90% and the molecular weight of this protein is around 190-240 kDa verified by SEC-MALS.
Report
Bioactivity-ELISA
Immobilized Cynomolgus ITGA6&ITGB4 Heterodimer Protein, His Tag&Tag Free (Cat. No. IN4-C52W3) at 5 μg/mL (100 μL/well) can bind Human Laminin 511 Protein, premium grade (Cat. No. LA8-H5283L) with a linear range of 0.2-8 ng/mL (QC tested).
Protocol
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FAQ
- product
Can lyophilized proteins remain stable during room-temperature shipping or temporary room-temperature exposure?
In general, lyophilized proteins remain stable during room-temperature shipping or temporary exposure to room-temperature conditions.
Many ACROBiosystems recombinant proteins are supplied in lyophilized (freeze-dried) form. The lyophilization process effectively removes moisture from the product, helping reduce degradation-related reactions and improve protein stability during transportation and storage.
To evaluate the stability of lyophilized proteins under normal temperature conditions, ACROBiosystems conducted a stability study on 11 representative lyophilized protein products at 37°C. The study demonstrated that these proteins maintained good quality stability after storage at 37°C for approximately 20 days. Based on these validation results, temporary room-temperature exposure during shipping or handling is generally not expected to have a significant impact on product quality or downstream application performance.
To ensure optimal long-term stability, products should be stored according to the storage conditions specified in the product Certificate of Analysis (COA) upon receipt.
Supporting Document
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