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Golden Hamster IL-23 alpha&IL-12 beta Heterodimer Protein, His Tag&Tag Free, low endotoxin (MALS verified)

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ILB-G52W4-50ug
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Product Details
Performance Data
Reviews & Citations
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  • Product Details
  • Performance Data
  • Reviews & Citations
  • Important Statement
  • Resources
Synonyms

IL-23 alpha & IL-12 beta

Source

Golden Hamster IL-23 alpha&IL-12 beta Heterodimer Protein, His Tag&Tag Free (ILB-G52W4) is expressed from human 293 cells (HEK293). It contains AA Leu 20 - Ala 196 (IL23A) & Ile 23 - Ser 327 (IL12B) (Accession # A0A1U7QS90 (IL23A) & Q8CJE6 (IL12B)).

Predicted N-terminus: Leu 20 (IL23A) & Ile 23 (IL12B)

Molecular Characterization
Other Tags and Version Biotin & Other Labeled Version

The protein has a calculated MW of 21.6 kDa (IL23A) & 34.8 kDa (IL12B). The protein migrates as 21-25 kDa and 38-40 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

The protein is designed as a heterodimer.

Endotoxin

Less than 0.01 EU per μg by the LAL method / rFC method.

Purity

>95% as determined by SDS-PAGE.

>95% as determined by SEC-MALS.

Formulation

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

Reconstitution

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Shipping and Storage

This product is shipped at ambient temperature.

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:
  • -20°C to -70°C for 12 months in lyophilized state;
  • -70°C for 3 months under sterile conditions after reconstitution.
ACRO Quality Management System

Background

Interleukin-23 subunit alpha (IL-23A) can associate with interleukin-12 subunit beta (IL-12B) to form IL-23, a heterodimeric cytokine that functions in innate and adaptive immunity. IL-23 promotes the production of IL-17, and together they contribute to acute responses to infection in peripheral tissues. IL-23 binds to a heterodimeric receptor complex composed of IL12RB1 and IL23R, activates the JAK-STAT signaling cascade, stimulates memory T-cells rather than naive T-cells, and promotes the production of proinflammatory cytokines. IL-23 induces autoimmune inflammation and thus may play a key role in autoimmune inflammatory diseases; it may also promote tumorigenesis in certain inflammatory contexts.