Human IL-34 Protein, His Tag, low endotoxin

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IL4-H52H3-100ug
$360.00
IL4-H52H3-1mg (250ug X 4)
$2615.00
ETA of in-stock products:2 business days
총 항목 수 제품 금액$ 0

제품 세부정보

  • Synonyms

    IL34, C16orf77, IL-34, Interleukin-34, MGC34647

  • Source

    Human IL-34, His Tag (IL4-H52H3) is expressed from human 293 cells (HEK293). It contains AA Asn 21 - Pro 242 (Accession # Q6ZMJ4-1).

    Request for sequence
  • Molecular Characterization

    IL-34 Structure

    Other Tags and Version Biotin & Other Labeled Version

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 27.3 kDa. The protein migrates as 32 kDa and 35-40 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin

    Less than 0.01 EU per μg by the LAL method / rFC method.

  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, 0.2 M Arginine, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Shipping and Storage

    This product is shipped at ambient temperature.

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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데이터 표시

  • SDS-PAGE

    IL-34 SDS-PAGE

    Human IL-34, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.

  • Bioactivity-ELISA

     IL-34 ELISA

    Immobilized Human M-CSF R, Mouse IgG2a Fc Tag (MALS verified) (Cat. No. CSR-H5255) at 5 μg/mL (100 μL/well) can bind Human IL-34, His Tag (Cat. No. IL4-H52H3) with a linear range of 0.4-2 ng/mL (QC tested).

    Protocol
  •  IL-34 ELISA

    Immobilized Human TREM2, Fc Tag (Cat. No. TR2-H5254) at 5 μg/mL (100 μL/well) can bind Human IL-34, His Tag (Cat. No. IL4-H52H3) with a linear range of 0.1-1 ng/mL (Routinely tested).

    Protocol

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배경 소개

nterleukins (IL) are a group of cytokines that play an important role in the immune system. They modulate inflammation and immunity by regulating growth, mobility and differentiation of lymphoid and other cells.This entry represents interleukin-34 (IL-34), it was identified via functional screening of a library of secreted proteins [1]. This cytokine promotes the differentiation and viability of monocytes and macrophages through the colony-stimulating factor-1 receptor (CSF1R)

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