Human IL-27 Protein, His Tag, premium grade (Liquid)

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Cat. No. / Size
Price
Qty
IL7-H5328-100ug
$360.00
ETA of in-stock products:2 business days

Product Details

  • Synonyms

    Interleukin-27, IL27A&IL27B, EBI3&p28

  • Source

    Human IL-27 Protein, His Tag, premium grade (Liquid) (IL7-H5328) is expressed from CHO cells. It contains AA Phe 29 - Pro 243 & Arg 21 - Lys 229 (Accession # Q14213-1 & Q8NEV9-1).

    Predicted N-terminus: Phe 29

    It is produced under our rigorous quality control system that incorporates a comprehensive set of tests including sterility and endotoxin tests. Product performance is carefully validated and tested for compatibility for cell culture use or any other applications in the early preclinical stage. When ready to transition into later clinical phases, we also offer a custom GMP protein service that tailors to your needs. We will work with you to customize and develop a GMP-grade product in accordance with your requests that also meets the requirements for raw and ancillary materials use in cell manufacturing of cell-based therapies.

  • Molecular Characterization

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 48.6 kDa. The protein migrates as 52 kDa±3 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin

    Less than 0.01 EU per μg by the LAL method / rFC method.

  • Host Cell DNA

    <0.02 ng/μg of protein tested by qPCR.

  • Sterility

    Negative

  • Mycoplasma

    Negative

  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Supplied as 0.2 μm filtered solution in PBS, pH7.4 with glycerol as protectant.

    Contact us for customized product form or formulation.

  • Shipping

    This product is supplied and shipped with dry ice, please inquire the shipping cost.

  • Storage

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. The product MUST be stored at -70°C or lower upon receipt;
    2. -70°C for 3 months under sterile conditions.
  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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Performance Data

  • SDS-PAGE

    IL-27 SDS-PAGE

    Human IL-27 Protein, His Tag, premium grade (Liquid) on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).

  • Bioactivity-CELL BASE

     IL-27 CELL

    Human IL-27 Protein, His Tag, premium grade (Liquid) (Cat. No. IL7-H5328) induced STAT3 (Luc) Reporter activity in HEK293 cells. The EC50 for this effect is 0.1175 μg/mL (Routinely tested).

    Protocol
  • Bioactivity-ELISA

     IL-27 ELISA

    Immobilized Human IL-27 Protein, His Tag, premium grade (Liquid) (Cat. No. IL7-H5328) at 2 μg/mL (100 μL/well) can bind Human IL-27 Ra, Fc Tag (Cat. No. ILA-H5254) with a linear range of 0.001-0.125 μg/mL (QC tested).

    Protocol

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Background

Interleukin-27 (IL-27) is a heterodimeric cytokine belonging to the IL-12 family that is composed of two subunits, Epstein-Barr virus (EBV)-induced gene 3 (EBI3) (also known as IL-27B) and IL27-p28 (known as IL-30). IL-27 is produced by antigen-presenting cells. IL-27 plays an important function in regulating the activity of B and T lymphocytes. The effects of IL-27 are eliciting by its interaction with a specific cell surface receptor complex composed of two proteins known as IL27R and gp130. IL-27 is a cytokine with pro- and anti-inflammatory properties, that can regulate T helper cell development, suppress T-cell proliferation, stimulate cytotoxic T cell activity, induce isotype switching in B-cells, and that has diverse effects on innate immune cells. Among its target cells are CD4 T helper cells which can differentiate in type 1 effector cells (TH1), type 2 effector cells (TH2) and IL17 producing helper T-cells (TH17). It drives rapid clonal expansion of naive but not memory CD4 T-cells. IL-27 reveals to be a potent inhibitor of TH17 cell development and of IL-17 production. IL-27 also antagonizes the effects of some cytokines such as IL6 through direct effects on T cells. Another important role of IL-27 is its antitumor activity and antiangiogenic activity.

Recent Advances

 
Drug Development Progress
  • English Name:

    Interleukin-27

  • Category:

  • Approved Drugs:

    0 Details

  • Drugs in Clinical Trials:

    1 Details

  • Highest Development Stage:

    Phase 2 Clinical

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