Biotinylated Human IL-2 R beta&IL-2 R alpha&IL-2 R gamma Protein, His,Avitag™&Twin Strep Tag (MALS verified)

Synonyms

IL-2 R beta & IL-2 R alpha & IL-2 R gamma

Source

Biotinylated Human IL-2RB&IL-2RA&IL-2RG, His,Avitag&Twin Strep Tag (ILG-H82W9) is expressed from human 293 cells (HEK293). It contains AA Ala 27 - Asp 239 (IL-2RB) & Glu 22 - Ser 212 (IL-2RA) & Leu 23 - Asn 254 (IL-2RG) (Accession # P14784-1 (IL-2RB) & P01589-1 (IL-2RA) & P31785-1 (IL-2RG)).

Predicted N-terminus: Ala 27 (IL-2RB) & Glu 22 (IL-2RA)

Molecular Characterization

Biotinylated Human IL-2RB&IL-2RA&IL-2RG, His,Avitag&Twin Strep Tag is produced by co-expression of IL-2RB and IL-2RA and IL-2RG, has a calculated MW of 31.5 kDa (IL-2RB) & 55.8 kDa (IL-2RA&IL-2RG). Subunit IL-2RB is fused with a his tag at the C-terminus, followed by a Avi tag (Avitag™) and subunit IL-2RA&IL-2RG is fused with a Twin-Strep Tag at the C-terminus. The reducing (R) protein migrates as 44 kDa,46 kDa and 80-90 kDa respectively due to glycosylation.

The protein is designed as a heterotrimer.

Labeling

Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

Protein Ratio

Passed as determined by the HABA assay / binding ELISA.

Purity

>90% as determined by SDS-PAGE.

Formulation

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

Reconstitution

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Shipping and Storage

This product is shipped at ambient temperature.

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

1. -20°C to -70°C for 12 months in lyophilized state;
2. -70°C for 3 months under sterile conditions after reconstitution.

ACRO Quality Management System

1. QMS(ISO, GMP)
2. Quality Advantages
3. Quality Control Process