Biotinylated Cynomolgus IL-13 Protein, His,Avitag™ (MALS verified)

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Price
Availability
Qty
IL3-C82E4-25ug
$415.00
In Stock - Delivery in 2-3 business days
IL3-C82E4-200ug
$1880.00
In Stock - Delivery in 2-3 business days

Product Details

  • Synonyms

    IL13, ALRH, BHR1, MGC116786, MGC116788, MGC116789, P600, Interleukin-13

  • Source

    Biotinylated Cynomolgus IL-13 Protein, His,Avitag (IL3-C82E4) is expressed from human 293 cells (HEK293). It contains AA Ser 21 - Asn 132 (Accession # ABG75889.1).

    Predicted N-terminus: Ser 21

    Request for sequence
  • Molecular Characterization

    IL-13 Structure

    Other Tags and Version Biotin & Other Labeled Version

    This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).

    The protein has a calculated MW of 15.9 kDa. The protein migrates as 13-15 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE).

  • Labeling

    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

  • Protein Ratio

    Passed as determined by the SABA assay / HABA assay / binding ELISA.

  • Purity

    >95% as determined by SDS-PAGE.

    >95% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Shipping and Storage

    This product is shipped at ambient temperature.

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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Performance Data

  • SDS-PAGE

    IL-13 SDS-PAGE

    Biotinylated Cynomolgus IL-13 Protein, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).

  • SEC-MALS

    IL-13 SEC-MALS

    The purity of Biotinylated Cynomolgus IL-13 Protein, His,Avitag (Cat. No. IL3-C82E4) is more than 95% and the molecular weight of this protein is around 15-20 kDa verified by SEC-MALS.

    Report
  • Bioactivity-ELISA

     IL-13 ELISA

    Immobilized Human IL-13 R alpha 2 Protein, Fc Tag (Cat. No. IL2-H5256) at 5 μg/mL (100 μL/well) can bind Biotinylated Cynomolgus IL-13 Protein, His,Avitag (Cat. No. IL3-C82E4) with a linear range of 0.005-1 ng/mL (QC tested).

    Protocol
  • Bioactivity-SPR

     IL-13 SPR

    Cynomolgus IL-13 R alpha 1, His Tag (Cat. No. IL1-C52H8) immobilized on CM5 Chip can bind Biotinylated Cynomolgus IL-13 Protein, His,Avitag (Cat. No. IL3-C82E4) with an affinity constant of 24.6 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).

    Protocol

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FAQ

  • product

    Can lyophilized proteins remain stable during room-temperature shipping or temporary room-temperature exposure?

    In general, lyophilized proteins remain stable during room-temperature shipping or temporary exposure to room-temperature conditions.


    Many ACROBiosystems recombinant proteins are supplied in lyophilized (freeze-dried) form. The lyophilization process effectively removes moisture from the product, helping reduce degradation-related reactions and improve protein stability during transportation and storage.


    To evaluate the stability of lyophilized proteins under normal temperature conditions, ACROBiosystems conducted a stability study on 11 representative lyophilized protein products at 37°C. The study demonstrated that these proteins maintained good quality stability after storage at 37°C for approximately 20 days. Based on these validation results, temporary room-temperature exposure during shipping or handling is generally not expected to have a significant impact on product quality or downstream application performance.


    To ensure optimal long-term stability, products should be stored according to the storage conditions specified in the product Certificate of Analysis (COA) upon receipt.


    Supporting Document


Background

Interleukin 13 (IL13) is also known as ALRH, BHR1and P600, is a single-chain glycosylated polypeptide, and is a cytokine critical in regulating inflammatory and immune responses. IL13 is secreted by many cell types, but especially by T helper type 2 (Th2) cells. IL-13 induces its effects through a multi-subunit receptor that includes the alpha chain of the IL-4 receptor (IL-4Rα) and at least one of two known IL-13-specific binding chains. The functions of IL-13 overlap considerably with those of IL-4, especially with regard to changes induced on hematopoietic cells, but these effects are probably less important given the more potent role of IL-4. IL-13 induces matrix metalloproteinases (MMPs) as part of a mechanism that protects against excessive allergic inflammation that predisposes to asphyxiation. IL-13 induces many features of allergic lung disease, including airway hyperresponsiveness, goblet cell metaplasia and mucus hypersecretion, which all contribute to airway obstruction.

Recent Advances

 
Drug Development Progress
  • English Name:

    Interleukin-13

  • Category:

  • Approved Drugs:

    4 Details

  • Drugs in Clinical Trials:

    27 Details

  • Highest Development Stage:

    Approved

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