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Product Details
Synonyms
IGF-I, IGF1A, somatomedin C, MGF
Source
Human IGF-I Protein, premium grade (IG1-H5117) is expressed from E. coli cells. It contains AA Gly 49 - Ala 118 (Accession # P05019-1).
Predicted N-terminus: Gly 49
It is produced under our rigorous quality control system that incorporates a comprehensive set of tests including sterility and endotoxin tests. Product performance is carefully validated and tested for compatibility for cell culture use or any other applications in the early preclinical stage. When ready to transition into later clinical phases, we also offer a custom GMP protein service that tailors to your needs. We will work with you to customize and develop a GMP-grade product in accordance with your requests that also meets the requirements for raw and ancillary materials use in cell manufacturing of cell-based therapies.
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries no "tag".
The protein has a calculated MW of 7.7 kDa. The protein migrates as 8 kDa±3 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE).
Endotoxin
Less than 0.01 EU per μg by the LAL method / rFC method.
Host Cell Protein
<0.5 ng/µg of protein tested by ELISA.
Host Cell DNA
<0.02 ng/μg of protein tested by qPCR.
Sterility
Negative
Mycoplasma
Negative
Purity
>95% as determined by SDS-PAGE.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
ACRO Quality Management System
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Performance Data
SDS-PAGE
Human IGF-I Protein, premium grade on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
Bioactivity-CELL BASE
Human IGF-I Protein, premium grade (Cat. No. IG1-H5117) stimulate Human IGF-1 R (Luc) HEK293 Reporter Cell. The specific activity of Human IGF-I Protein, premium grade is > 3.00 x 10^2 IU/mg, which is calibrated against human IGF-I WHO International Standard (NIBSC code: 91/554) (QC tested).
Protocol
Bioactivity-ELISA
Immobilized Human IGF-I Protein, premium grade (Cat. No. IG1-H5117) at 5 μg/mL (100 μL/well) can bind Human IGF-I R, Fc Tag (Cat. No. IGR-H5253) with a linear range of 0.06-4 μg/mL (Routinely tested).
Protocol
Bioactivity-SPR
Biotinylated Human IGF-I R Protein, His,Avitag (Cat. IGR-H83E3) immobilized on SA Chip can bind Human IGF-I Protein, premium grade (Cat. No. IG1-H5117) with an affinity constant of 9.16 nM as determined in a SPR assay (Biacore 8K) (QC tested).
Protocol
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Background
Recent Advances
Drug Development Progress- English Name：
Insulin-like growth factor I
- Category：
- Approved Drugs：
0 Details
- Drugs in Clinical Trials：
9 Details
- Highest Development Stage：
Phase 3 Clinical
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