Biotinylated Mouse IFN-beta Protein, His,Avitag™ (MALS & SPR verified)

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IFB-M82E3-25ug
$360.00
In Stock - Delivery in 2-3 business days
IFB-M82E3-200ug
$1420.00
In Stock - Delivery in 2 weeks
총 항목 수 제품 금액$ 0

제품 세부정보

  • Synonyms

    IFNB1, Interferon beta, IFN-beta, IFB, IFNB

  • Source

    Biotinylated Mouse IFN-beta Protein, His,Avitag (IFB-M82E3) is expressed from human 293 cells (HEK293). It contains AA Ile 22 - Asn 182 (Accession # P01575).

    Request for sequence
  • Molecular Characterization

    IFN-beta Structure

    Other Tags and Version Biotin & Other Labeled Version

    This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).

    The protein has a calculated MW of 23.6 kDa. The protein migrates as 33-38 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Labeling

    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

  • Protein Ratio

    Passed as determined by the HABA assay / binding ELISA.

  • Purity

    >95% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Shipping and Storage

    This product is shipped at ambient temperature.

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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데이터 표시

  • SDS-PAGE

    IFN-beta SDS-PAGE

    Biotinylated Mouse IFN-beta Protein, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).

  • SEC-MALS

    IFN-beta SEC-MALS

    The purity of Biotinylated Mouse IFN-beta Protein, His,Avitag (Cat. No. IFB-M82E3) is more than 90% and the molecular weight of this protein is around 35-50 kDa verified by SEC-MALS.

    Report
  • Bioactivity-SPR

     IFN-beta SPR

    Biotinylated Mouse IFN-beta Protein, His,Avitag (Cat. No. IFB-M82E3) captured on Biotin CAP-Series S Sensor Chip can bind Mouse IFN-alpha / beta R1, His Tag (Cat. No. IF1-M5225) with an affinity constant of 0.277 nM as determined in a SPR assay (Biacore 8K) (QC tested).

    Protocol

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배경 소개

Interferon beta(IFN beta) is type I interferon cytokine that plays a key role in the innate immune response to infection, developing tumors and other inflammatory stimuli. Signals via binding to high-affinity (IFR2) and low-affinity (IFR1) heterodimeric receptor, activating the canonical Jak-STAT signaling pathway resulting in transcriptional activation or repression of interferon-regulated genes that encode the effectors of the interferon response, such as antiviral proteins, regulators of cell proliferation and differentiation, and immunoregulatory proteins. IFNB1 is more potent than interferon-alpha (IFN-alpha) in inducing the apoptotic and antiproliferative pathways required for control of tumor cell growth (By similarity).

프론티어 진행

 
의약품 개발 진행
  • 영어 이름:

    Interferon beta

  • 카테고리:

  • 상장 의약품 수:

    1 상세

  • 임상 의약품 수:

    6 상세

  • 최고 개발 단계:

    Approved

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