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Biotinylated Human IFN-alpha 1 Protein, His,Avitag™ (HPLC & SPR verified)

AIDD‑Ready
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IFA-H82E4-25ug
$360.00
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IFA-H82E4-200ug
$1,420.00
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IFA-H82E4
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Product Details
Performance Data
Reviews & Citations
Resources
  • Product Details
  • Performance Data
  • Reviews & Citations
  • Important Statement
  • Resources
Synonyms

IFN-alpha 1, IFNA1, IFNalpha 1, IFN-alpha-1, LeIF D

Source

Biotinylated Human IFN-alpha 1 Protein, His,Avitag (IFA-H82E4) is expressed from human 293 cells (HEK293). It contains AA Cys 24 - Glu 189 (Accession # P01562).

Predicted N-terminus: Cys 24

Molecular Characterization
Other Tags and Version Biotin & Other Labeled Version

This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).

The protein has a calculated MW of 23.1 kDa. The protein migrates as 23-26 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

Labeling

Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

Protein Ratio

Passed as determined by the SABA assay / HABA assay / binding ELISA.

Purity

>95% as determined by SDS-PAGE.

>90% as determined by SEC-HPLC.

Formulation

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

Reconstitution

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Shipping and Storage

This product is shipped at ambient temperature.

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:
  • -20°C to -70°C for 12 months in lyophilized state;
  • -70°C for 3 months under sterile conditions after reconstitution.
ACRO Quality Management System

Background

Interferon alpha-1 is also known as IFN-alpha-1/13, Interferon alpha-D, LeIF D and IF1, belongs to the alpha / beta interferon family. Interferons alpha-1 and alpha-13 have identical protein sequences. Produced by macrophages, IFN-alpha have antiviral activities. Interferon stimulates the production of two enzymes: a protein kinase and an oligoadenylate synthetase. IFN-alpha can either suppress or promote the development of autoimmune diabetes. It is likely that IFN-alpha plays a complex role in the etiology of type 1 diabetes.