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Synonyms
Hyaluronidase PH-20
Source
Human Hyaluronidase PH-20 Protein, Tag Free (PH0-H5219) is expressed from human 293 cells (HEK293). It contains AA Leu 36 - Ser 490 (Accession # P38567-1).
Predicted N-terminus: Leu 36
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries no "tag".
The protein has a calculated MW of 52.6 kDa. The protein migrates as 65-70 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin
Less than 0.1 EU per μg by the LAL method / rFC method.
Purity
>95% as determined by SDS-PAGE.
>95% as determined by SEC-MALS.
Formulation
Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM NaCl, pH7.5 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Shipping and Storage
This product is shipped at ambient temperature.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
ACRO Quality Management System
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SDS-PAGE
Human Hyaluronidase PH-20 Protein, Tag Free on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
SEC-MALS
The purity of Human Hyaluronidase PH-20 Protein, Tag Free (Cat. No. PH0-H5219) is more than 95% and the molecular weight of this protein is around 53-71 kDa verified by SEC-MALS.
Report
Bioactivity
The activity of Hyaluronidase PH-20 is measured by its ability to hydrolyze HA in turbidimetric assay (45 minute assay). The specific activity is >55000 U/mg. (Unit Definition: One unit of Hyaluronidase activity will cause a change in A600 of 0.330 per minute at pH5.35 at 37 °C in a 2.0 mL reaction mixture) (QC tested).
Protocol
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E***l2023-01-14
좋아요The timely fashion in which orders are completed and the quick access to a variety of products makes returning back to Acro a promising investment. Ordering the product PH0-H5219-200ug was a simple and easy task to complete, especially with Acro’s user-friendly website. Delivery of the items as well, with Acro, as long as the product is available it arrives in a timely fashion. I also like the way the items are packaged and that documentation can be found quickly for each product.
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