Product Details
Synonyms
GHR, GHBP, GH receptor
Source
MABSol® Biotinylated Human GHR Protein, His Tag, primary amine labeling (GHR-H8222) is expressed from human HEK293 cells. It contains AA Ala 27 - Tyr 264 (Accession # P10912-1). It is the biotinylated form of Human GHR, His Tag (Cat. No. GHR-H5222).
Predicted N-terminus: Ala 27
Request for sequenceMolecular Characterization

Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 29.6 kDa. The protein migrates as 38-55 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
Labeling
The primary amines in the side chains of lysine residues and the N-terminus of the protein are conjugated with biotins using standard chemical labeling method. A standard biotin reagent (13.5 angstroms) is used in this product.
Protein Ratio
Passed as determined by the HABA assay / binding ELISA.
Purity
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Performance Data
SDS-PAGE

Biotinylated Human GHR Protein, His Tag, primary amine labeling on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS

The purity of Biotinylated Human GHR Protein, His Tag, primary amine labeling (Cat. No. GHR-H8222) is more than 90% and the molecular weight of this protein is around 42-60 kDa verified by SEC-MALS.
Report
Bioactivity-ELISA

Immobilized Human GH, premium grade (Cat. No. GH1-H4118) at 2 μg/mL (100 μL/well) can bind Monoclonal Anti-Human Claudin 4 antibody, Human IgG1 (Cat. No. GHR-H8222) with a linear range of 0.06-1 ng/mL (QC tested).
Protocol
Customer Reviews Writing Reviews

Background
Recent Advances
- English Name:
Growth hormone receptor
- Category:
- Approved Drugs:
29 Details
- Drugs in Clinical Trials:
31 Details
- Highest Development Stage:
Approved
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