Canine Growth Hormone R (GHR) Protein, His Tag

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Cat. No. / Size
Price
Qty
GHR-C52H9-100ug
$360.00
GHR-C52H9-1mg (500ug X 2)
$2380.00
ETA of in-stock products:2 business days

Product Details

  • Synonyms

    GHR, GHBP, GH receptor

  • Source

    Canine Growth Hormone R, His Tag (GHR-C52H9) is expressed from human 293 cells (HEK293). It contains AA Thr 27 - Gln 264 (Accession # Q9TU69-1).

    Predicted N-terminus: Thr 27

    Request for sequence
  • Molecular Characterization

    Growth Hormone R Structure

    Other Tags and Version Biotin & Other Labeled Version

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 29.2 kDa. The protein migrates as 43-53 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin

    Less than 1.0 EU per μg by the LAL method / rFC method.

  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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Performance Data

  • SDS-PAGE

    Growth Hormone R SDS-PAGE

    Canine Growth Hormone R, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.

  • Bioactivity-ELISA

     Growth Hormone R ELISA

    Immobilized Human GH, Tag Free at 2 μg/mL (100 μL/well) can bind Canine Growth Hormone R, His Tag (Cat. No. GHR-C52H9) with a linear range of 0.1-16 ng/mL (QC tested).

    Protocol
  • Bioactivity-BLI

     Growth Hormone R BLI

    Loaded Canine Growth Hormone R, His Tag (Cat. No. GHR-C52H9) on HIS1K Biosensor, can bind Human GH, Tag Free with an affinity constant of 0.404 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

    Protocol

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Background

Growth hormone receptor (GHR) is also known as somatotropin receptor, growth hormone-binding protein (GHBR), which belongs to the type I cytokine receptor family or Type 1 subfamily. GHR contains one fibronectin type-III domain. GHR / GHBR is expressed in various tissues with high expression in liver and skeletal muscle. The soluble form (GHBP) is produced by phorbol ester-promoted proteolytic cleavage at the cell surface (shedding) by ADAM17/TACE. GHR is receptor for pituitary gland growth hormone involved in regulating postnatal body growth. On ligand binding, couples to the JAK2/STAT5 pathway. The soluble form (GHBP) acts as a reservoir of growth hormone in plasma and may be a modulator/inhibitor of GH signaling.

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Drug Development Progress
  • English Name:

    Growth hormone receptor

  • Category:

  • Approved Drugs:

    29 Details

  • Drugs in Clinical Trials:

    31 Details

  • Highest Development Stage:

    Approved

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