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GDF-8, Myostatin, GDF8, MSTN, Growth, differentiation factor 8
Human latent GDF-8, His Tag (GD8-H5243) is expressed from human 293 cells (HEK293). It contains AA Asn 24 - Ser 375 (Accession # O14793-1).
Predicted N-terminus: His
This protein carries a polyhistidine tag at the N-terminus. Pro-GDF8 was predominantly migrated to 45-50 kDa precursor monomers, and latent GDF8 showed 35 kDa prodomain monomer bands together with a 14 kDa growth factor monomer when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE).
The protein is designed as a dimer.
Human latent GDF-8, His Tag(GD8-H5243) was digested with trypsin and characterized by LC-MS/MS peptide mapping, achieving 98.7% sequence coverage (Routinely tested).
Less than 0.01 EU per μg by the LAL method / rFC method.
>90% as determined by SDS-PAGE.
>95% as determined by SEC-MALS.
Lyophilized from 0.22 μm filtered solution in PBS, 0.2 M Arginine, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
This product is shipped at ambient temperature.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
