Biotinylated Cynomolgus / Rhesus macaque FOLR1 Protein, His,Avitag™ (Folic Acid Complex, MALS verified)

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제품 번호/규격
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FO1-C82E9-25ug
$368.00
FO1-C82E9-200ug
$1450.00
ETA of in-stock products:2 business days
총 항목 수 제품 금액$ 0

제품 세부정보

  • Synonyms

    FOLR-1, FBP, FOLR, FRα

  • Source

    Biotinylated Cynomolgus / Rhesus macaque FOLR1 Protein, His,Avitag (FO1-C82E9) is expressed from human 293 cells (HEK293). It contains AA Arg 25 - Met 233 (Accession # NP_001181576.1). In the region Arg 25 - Met 233, the amino acid sequences of Cynomolgus and Rhesus macaque FOLR1 are identical.

    Predicted N-terminus: Arg 25

    Request for sequence
  • Molecular Characterization

    FOLR1 Structure

    Other Tags and Version Biotin & Other Labeled Version

    This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).

    The protein has a calculated MW of 28.1 kDa. The protein migrates as 33-43 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Labeling

    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

  • Protein Ratio

    Passed as determined by the HABA assay / binding ELISA.

  • Endotoxin

    Less than 1.0 EU per μg by the LAL method / rFC method.

  • Purity

    >90% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Shipping and Storage

    This product is shipped at ambient temperature.

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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데이터 표시

  • SDS-PAGE

    FOLR1 SDS-PAGE

    Biotinylated Cynomolgus / Rhesus macaque FOLR1 Protein, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).

  • SEC-MALS

    FOLR1 SEC-MALS

    The purity of Biotinylated Cynomolgus / Rhesus macaque FOLR1 Protein, His,Avitag (Cat. No. FO1-C82E9) is more than 90% and the molecular weight of this protein is around 30-45 kDa verified by SEC-MALS.

    Report
  • Bioactivity-ELISA

     FOLR1 ELISA

    Immobilized Folic acid -BSA conjugate at 5 μg/mL (100 μL/well) can bind Biotinylated Cynomolgus / Rhesus macaque FOLR1 Protein, His,Avitag (Cat. No. FO1-C82E9) with a linear range of 0.001-0.25 μg/mL (QC tested).

    Protocol
  •  FOLR1 ELISA

    Immobilized Biotinylated Cynomolgus / Rhesus macaque FOLR1 Protein, His,Avitag (Cat. No. FO1-C82E9) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Mirvetuximab with a linear range of 0.03-1 ng/mL (QC tested).

    Protocol

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배경 소개

Folate Receptor 1 (FOLR1) is also known as Folate receptor alpha, Folate Binding Protein (FBP), FOLR, and is a member of the folate receptor (FOLR) family. Members of this gene family have a high affinity for folic acid and for several reduced folic acid derivatives, and mediate delivery of 5-methyltetrahydrofolate to the interior of cells. Mature FOLR1 is an N-glycosylated protein that is anchored to the cell surface by a GPI linkage. FOLR1 is predominantly expressed on epithelial cells and is dramatically upregulated on many carcinomas. FOLR1 is internalized to the endosomal system where it dissociates from its ligand before recycling to the cell surface. A soluble form of FOLR1 can be proteolytically shed from the cell surface into the serum and breast milk. Defects in FOLR1 are the cause of neurodegeneration due to cerebral folate transport deficiency (NCFTD). NCFTD is an autosomal recessive disorder resulting from brain-specific folate deficiency early in life.

프론티어 진행

 
의약품 개발 진행
  • 영어 이름:

    Folate receptor alpha

  • 카테고리:

  • 상장 의약품 수:

    3 상세

  • 임상 의약품 수:

    27 상세

  • 최고 개발 단계:

    Approved

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